Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3109555..3110125 Replicon chromosome
Accession NZ_CP007027
Organism Mycobacterium tuberculosis H37RvSiena

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag Y980_RS14800 Protein ID WP_003414166.1
Coordinates 3109555..3109911 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag Y980_RS14805 Protein ID WP_003901465.1
Coordinates 3109895..3110125 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Y980_RS14780 3105007..3106695 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
Y980_RS14785 3106699..3107025 - 327 WP_003414157.1 hypothetical protein -
Y980_RS14790 3107198..3107785 + 588 WP_003914429.1 DUF3558 family protein -
Y980_RS14795 3107804..3109453 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
Y980_RS14800 3109555..3109911 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
Y980_RS14805 3109895..3110125 - 231 WP_003901465.1 antitoxin MazE Antitoxin
Y980_RS14810 3110168..3111211 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
Y980_RS22270 3111402..3111677 + 276 WP_003911993.1 DUF1778 domain-containing protein -
Y980_RS22440 3111853..3112107 - 255 WP_003917684.1 hypothetical protein -
Y980_RS14825 3112255..3112659 + 405 WP_009938577.1 hypothetical protein -
Y980_RS14830 3112656..3112847 + 192 WP_003414184.1 hypothetical protein -
Y980_RS14835 3112911..3114200 + 1290 Protein_2902 transposase family protein -
Y980_RS14840 3114434..3114691 + 258 WP_003899489.1 hypothetical protein -
Y980_RS14845 3114796..3115107 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T45929 WP_003414166.1 NZ_CP007027:c3109911-3109555 [Mycobacterium tuberculosis H37RvSiena]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T45929 NZ_CP007027:c3109911-3109555 [Mycobacterium tuberculosis H37RvSiena]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT45929 WP_003901465.1 NZ_CP007027:c3110125-3109895 [Mycobacterium tuberculosis H37RvSiena]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT45929 NZ_CP007027:c3110125-3109895 [Mycobacterium tuberculosis H37RvSiena]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References