Detailed information of TA system TA045928

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3069971..3070658
• Replicon: chromosome
• Accession: NZ_CP007027
• Organism: Mycobacterium tuberculosis H37RvSiena

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: Y980_RS14590
• Protein ID: WP_003414064.1
• Coordinates: 3070263..3070658 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: Y980_RS14585
• Protein ID: WP_003414061.1
• Coordinates: 3069971..3070237 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y980_RS14560	3065610..3066512	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
Y980_RS14565	3066581..3067333	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
Y980_RS14570	3067577..3067852	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
Y980_RS14575	3067849..3069471	-	1623	WP_003906897.1	type I restriction-modification system subunit M	-
Y980_RS14580	3069558..3069974	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
Y980_RS14585	3069971..3070237	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
Y980_RS14590	3070263..3070658	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
Y980_RS14595	3070655..3070924	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
Y980_RS14600	3070934..3072028	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
Y980_RS14605	3072025..3072444	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
Y980_RS14610	3072518..3072997	-	480	WP_003414073.1	dihydrofolate reductase	-
Y980_RS14615	3073068..3073868	-	801	WP_003911953.1	thymidylate synthase	-
Y980_RS14620	3074024..3074761	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T45928 WP_003414064.1 NZ_CP007027:c3070658-3070263 [Mycobacterium tuberculosis H37RvSiena]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T45928 NZ_CP007027:c3070658-3070263 [Mycobacterium tuberculosis H37RvSiena]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT45928 WP_003414061.1 NZ_CP007027:c3070237-3069971 [Mycobacterium tuberculosis H37RvSiena]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT45928 NZ_CP007027:c3070237-3069971 [Mycobacterium tuberculosis H37RvSiena]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64