Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2867994..2868625 Replicon chromosome
Accession NZ_CP007027
Organism Mycobacterium tuberculosis H37RvSiena

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag Y980_RS13495 Protein ID WP_003413174.1
Coordinates 2868248..2868625 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag Y980_RS13490 Protein ID WP_003413167.1
Coordinates 2867994..2868251 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Y980_RS13460 2863908..2864222 + 315 WP_009937839.1 hypothetical protein -
Y980_RS13465 2864518..2865729 + 1212 WP_009936235.1 alpha/beta hydrolase family protein -
Y980_RS13470 2865856..2866515 + 660 WP_003900846.1 LppA family lipoprotein -
Y980_RS13475 2866512..2867174 + 663 WP_003900848.1 hypothetical protein -
Y980_RS13480 2867171..2867449 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
Y980_RS13485 2867542..2867955 + 414 WP_003413164.1 PIN domain nuclease -
Y980_RS13490 2867994..2868251 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
Y980_RS13495 2868248..2868625 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
Y980_RS13500 2868641..2869015 - 375 WP_003413177.1 hypothetical protein -
Y980_RS13505 2869115..2869510 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
Y980_RS13510 2869507..2869752 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
Y980_RS13515 2870163..2870582 - 420 WP_003413190.1 A24 family peptidase -
Y980_RS13520 2870594..2871403 - 810 WP_003413193.1 shikimate dehydrogenase -
Y980_RS13525 2871400..2872653 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
Y980_RS13530 2872646..2873158 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T45923 WP_003413174.1 NZ_CP007027:2868248-2868625 [Mycobacterium tuberculosis H37RvSiena]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T45923 NZ_CP007027:2868248-2868625 [Mycobacterium tuberculosis H37RvSiena]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT45923 WP_003413167.1 NZ_CP007027:2867994-2868251 [Mycobacterium tuberculosis H37RvSiena]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT45923 NZ_CP007027:2867994-2868251 [Mycobacterium tuberculosis H37RvSiena]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References