Detailed information of TA system TA045921

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2853655..2854295
• Replicon: chromosome
• Accession: NZ_CP007027
• Organism: Mycobacterium tuberculosis H37RvSiena

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: Y980_RS13400
• Protein ID: WP_003412970.1
• Coordinates: 2853655..2854074 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: Y980_RS13405
• Protein ID: WP_003412975.1
• Coordinates: 2854071..2854295 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y980_RS13375	2849240..2849962	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
Y980_RS13380	2850479..2850706	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
Y980_RS13385	2850703..2851104	+	402	WP_003412963.1	PIN domain-containing protein	-
Y980_RS13390	2851139..2852059	-	921	WP_003412965.1	restriction endonuclease	-
Y980_RS22530	2852400..2852645	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
Y980_RS13395	2852704..2853654	+	951	WP_003911916.1	Lsr2 family protein	-
Y980_RS13400	2853655..2854074	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
Y980_RS13405	2854071..2854295	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
Y980_RS13410	2854326..2857169	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
Y980_RS13415	2857241..2857642	-	402	WP_003412981.1	hypothetical protein	-
Y980_RS13420	2857642..2858112	-	471	WP_003899364.1	transcription antitermination factor NusB	-
Y980_RS13425	2858115..2858678	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T45921 WP_003412970.1 NZ_CP007027:c2854074-2853655 [Mycobacterium tuberculosis H37RvSiena]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T45921 NZ_CP007027:c2854074-2853655 [Mycobacterium tuberculosis H37RvSiena]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT45921 WP_003412975.1 NZ_CP007027:c2854295-2854071 [Mycobacterium tuberculosis H37RvSiena]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT45921 NZ_CP007027:c2854295-2854071 [Mycobacterium tuberculosis H37RvSiena]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09