Detailed information of TA system TA045909

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2194032..2194735
• Replicon: chromosome
• Accession: NZ_CP007027
• Organism: Mycobacterium tuberculosis H37RvSiena

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: Y980_RS10195
• Protein ID: WP_003409778.1
• Coordinates: 2194032..2194361 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: Y980_RS21345
• Protein ID: WP_003409780.1
• Coordinates: 2194358..2194735 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y980_RS10175	2190415..2191485	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
Y980_RS10180	2191482..2191997	+	516	WP_003409718.1	flavin reductase family protein	-
Y980_RS10185	2191994..2193055	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
Y980_RS10190	2193052..2193822	+	771	WP_003409775.1	SDR family oxidoreductase	-
Y980_RS10195	2194032..2194361	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
Y980_RS21345	2194358..2194735	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
Y980_RS10205	2194732..2195322	-	591	WP_003409784.1	SEC-C domain-containing protein	-
Y980_RS10210	2195377..2196741	+	1365	WP_003903691.1	HNH endonuclease	-
Y980_RS10215	2196896..2197348	-	453	WP_003899095.1	lipoprotein	-
Y980_RS10220	2197412..2197813	+	402	WP_003409869.1	hypothetical protein	-
Y980_RS10225	2197806..2197988	-	183	WP_003409870.1	hypothetical protein	-
Y980_RS10230	2198102..2198452	-	351	WP_003899096.1	hypothetical protein	-
Y980_RS10235	2198463..2199365	-	903	WP_003899097.1	hypothetical protein	-
Y980_RS10240	2199386..2199577	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T45909 WP_003409778.1 NZ_CP007027:c2194361-2194032 [Mycobacterium tuberculosis H37RvSiena]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T45909 NZ_CP007027:c2194361-2194032 [Mycobacterium tuberculosis H37RvSiena]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT45909 WP_003409780.1 NZ_CP007027:c2194735-2194358 [Mycobacterium tuberculosis H37RvSiena]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT45909 NZ_CP007027:c2194735-2194358 [Mycobacterium tuberculosis H37RvSiena]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8