Detailed information of TA system TA045846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 4302896..4303484
• Replicon: chromosome
• Accession: NZ_CP007012
• Organism: Pseudomonas sp. FGI182

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: V9X090
• Locus tag: C163_RS18985
• Protein ID: WP_025340160.1
• Coordinates: 4302896..4303198 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: C163_RS18990
• Protein ID: WP_025340161.1
• Coordinates: 4303191..4303484 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C163_RS18965	4298991..4299884	-	894	WP_025340156.1	LysR family transcriptional regulator	-
C163_RS18970	4299991..4301163	+	1173	WP_025340157.1	MFS transporter	-
C163_RS18975	4301291..4302217	-	927	WP_025340158.1	DMT family transporter	-
C163_RS18980	4302324..4302719	-	396	WP_025340159.1	hypothetical protein	-
C163_RS18985	4302896..4303198	+	303	WP_025340160.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C163_RS18990	4303191..4303484	+	294	WP_025340161.1	putative addiction module antidote protein	Antitoxin
C163_RS18995	4303715..4304515	-	801	WP_025340162.1	helix-turn-helix transcriptional regulator	-
C163_RS19000	4304601..4305833	-	1233	WP_025340163.1	ethanolamine utilization protein EutH	-
C163_RS19005	4305932..4306696	-	765	WP_025340164.1	glucose 1-dehydrogenase	-
C163_RS19010	4306853..4307125	+	273	WP_025340165.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4294822..4305833	11011

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (5-95)

Antitoxin

• Uncharacterized protein HI1420 (5-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11290.93 Da
• Isoelectric Point: 10.8773

>T45846 WP_025340160.1 NZ_CP007012:4302896-4303198 [Pseudomonas sp. FGI182]
MKKIESSSFRHWVTGLRDVSARARIISRINRLMEGLPGDVSPVGHGVSELRVHYGPGYRVYFHQAGSTFVILLCGGDKGS
QQRDIKAAHQILRSWRMQND

Nucleotide

• Length: 303 bp

>T45846 NZ_CP007012:4302896-4303198 [Pseudomonas sp. FGI182]
ATGAAAAAAATCGAATCCAGCAGTTTCAGACATTGGGTGACCGGTCTCCGAGACGTTAGTGCCAGAGCCCGAATCATTTC
TCGGATCAACCGGTTGATGGAGGGCCTGCCTGGCGACGTATCACCGGTTGGCCACGGCGTCAGTGAGCTCAGGGTTCACT
ATGGGCCTGGCTACCGGGTGTACTTCCATCAGGCTGGCAGCACATTTGTCATTCTGCTTTGCGGCGGCGACAAAGGCAGC
CAACAGCGCGACATCAAGGCAGCCCACCAGATTCTGCGTTCGTGGAGGATGCAAAATGACTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10459.88 Da
• Isoelectric Point: 5.0495

>AT45846 WP_025340161.1 NZ_CP007012:4303191-4303484 [Pseudomonas sp. FGI182]
MTETFSDFDPAQYLTTPEAIAQFMTDALETGDAGYVAKAVGVVARAKGMSELAKETGLSREQLYRSFSEHGNPTLKSFLT
VMKALGIDMTARPHLAG

Nucleotide

• Length: 294 bp

>AT45846 NZ_CP007012:4303191-4303484 [Pseudomonas sp. FGI182]
ATGACTGAAACCTTCTCTGATTTCGACCCCGCCCAGTACCTCACTACCCCTGAAGCGATCGCGCAGTTCATGACCGACGC
ACTCGAAACCGGGGATGCCGGCTATGTCGCCAAGGCTGTAGGCGTGGTAGCGCGTGCAAAAGGCATGAGCGAGCTGGCTA
AAGAAACCGGTCTGTCGCGCGAGCAGCTCTATCGGTCTTTCAGTGAGCATGGCAACCCGACTCTGAAGTCGTTCCTGACC
GTGATGAAGGCGCTGGGAATAGACATGACGGCGCGGCCTCACCTCGCTGGCTGA

Structures

Toxin

• AlphaFold DB: V9X090