Detailed information of TA system TA045844

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 294024..294897
• Replicon: chromosome
• Accession: NZ_CP007012
• Organism: Pseudomonas sp. FGI182

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C163_RS01275
• Protein ID: WP_081742214.1
• Coordinates: 294024..294377 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C163_RS01280
• Protein ID: WP_025337351.1
• Coordinates: 294424..294897 (+)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C163_RS01260	290330..291049	-	720	WP_025337347.1	response regulator	-
C163_RS01265	291190..293487	+	2298	WP_025337348.1	TonB-dependent siderophore receptor	-
C163_RS01270	293640..293846	-	207	WP_025337349.1	DUF3079 domain-containing protein	-
C163_RS01275	294024..294377	+	354	WP_081742214.1	type II toxin-antitoxin system HigB family toxin	Toxin
C163_RS01280	294424..294897	+	474	WP_025337351.1	transcriptional regulator	Antitoxin
C163_RS26070	295282..295734	-	453	WP_081742215.1	hypothetical protein	-
C163_RS01290	295926..296111	-	186	Protein_248	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	293640..306660	13020

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (73-111)

Antitoxin

• Antitoxin HigA (43-154)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13678.86 Da
• Isoelectric Point: 10.4174

>T45844 WP_081742214.1 NZ_CP007012:294024-294377 [Pseudomonas sp. FGI182]
MRVITKAAVTKAIEAHAQWKAPLSLWLTTFDRATLRFESFEQLRNTWSTAFSWNVDRIPCSKLRPSSRKGPLDVYVFDIK
KNECRLIAWLNTRTGTLYIKAILSHAEYDKWCKSDVR

Nucleotide

• Length: 354 bp

>T45844 NZ_CP007012:294024-294377 [Pseudomonas sp. FGI182]
ATGCGGGTGATAACGAAAGCGGCGGTTACCAAAGCCATCGAAGCTCACGCCCAGTGGAAAGCCCCCCTGAGCCTGTGGCT
CACGACCTTTGATAGGGCGACGCTGCGCTTCGAGTCTTTTGAGCAGCTAAGGAATACGTGGTCTACCGCCTTTAGCTGGA
ACGTTGACCGCATTCCTTGTTCAAAACTCAGGCCCTCCAGCCGAAAAGGGCCGCTTGATGTCTATGTGTTCGATATCAAG
AAAAACGAATGCCGTCTGATCGCATGGCTCAACACACGAACGGGCACCCTCTATATCAAAGCCATTCTGTCGCACGCGGA
GTACGACAAGTGGTGCAAGAGCGATGTCAGGTAG

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17228.68 Da
• Isoelectric Point: 4.5725

>AT45844 WP_025337351.1 NZ_CP007012:294424-294897 [Pseudomonas sp. FGI182]
MKRDKIEANGLDAFIANISPMLDGLNAQMATMAQLLAACHDPIKDDAELQARMALIDELYSHADSESHAAARFAEMVADR
VYEYESESVLVPFASQAEALAFLLSDRGVKQKDLASIATQSAVSEILNNKRKMTVAQIKGFAEFFSVPVEFFMHGVV

Nucleotide

• Length: 474 bp

>AT45844 NZ_CP007012:294424-294897 [Pseudomonas sp. FGI182]
ATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATCTCACCCATGCTGGATGGCCTGAACGC
CCAGATGGCAACCATGGCCCAGCTTCTTGCTGCCTGCCATGACCCTATCAAGGACGATGCAGAGCTCCAGGCGCGGATGG
CATTGATCGATGAGCTCTACTCGCATGCCGACAGCGAAAGTCACGCAGCAGCGCGCTTTGCGGAGATGGTGGCGGATCGT
GTCTACGAGTATGAGAGTGAGTCGGTACTCGTGCCGTTTGCGAGCCAGGCCGAAGCGTTGGCCTTCCTGCTGTCGGACAG
GGGCGTGAAGCAGAAGGACCTTGCCTCGATAGCAACTCAGAGCGCGGTTTCAGAGATTTTGAACAACAAGCGGAAGATGA
CGGTTGCCCAGATAAAGGGCTTTGCTGAGTTTTTCAGCGTACCGGTTGAGTTTTTCATGCATGGGGTGGTTTGA