Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-Phd
Location 661582..662267 Replicon plasmid pRetIE4771e
Accession NZ_CP006991
Organism Rhizobium sp. IE4771

Toxin (Protein)


Gene name vapC Uniprot ID A0A060IDY5
Locus tag IE4771_RS32790 Protein ID WP_040113056.1
Coordinates 661845..662267 (+) Length 141 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A060IA78
Locus tag IE4771_RS32785 Protein ID WP_040143124.1
Coordinates 661582..661848 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IE4771_RS32765 657197..658249 + 1053 WP_040113051.1 iron ABC transporter permease -
IE4771_RS32770 658246..659034 + 789 WP_040143121.1 ABC transporter ATP-binding protein -
IE4771_RS32775 659063..659491 - 429 WP_040113053.1 MarR family transcriptional regulator -
IE4771_RS32780 659698..661509 + 1812 WP_040143122.1 chloride channel protein -
IE4771_RS32785 661582..661848 + 267 WP_040143124.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
IE4771_RS32790 661845..662267 + 423 WP_040113056.1 type II toxin-antitoxin system VapC family toxin Toxin
IE4771_RS32795 662293..663063 - 771 WP_040143125.1 ABC transporter ATP-binding protein -
IE4771_RS32800 663073..664056 - 984 WP_040143127.1 ABC transporter substrate-binding protein -
IE4771_RS32805 664095..664946 - 852 WP_009992575.1 ABC transporter permease -
IE4771_RS32810 664957..666309 - 1353 WP_040113060.1 LLM class flavin-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..744104 744104


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-125)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(9-63)


Sequences

Toxin        


Download         Length: 141 a.a.        Molecular weight: 15617.02 Da        Isoelectric Point: 4.6791

>T45839 WP_040113056.1 NZ_CP006991:661845-662267 [Rhizobium sp. IE4771]
VRLLLDTNVLLEVTKPNPEMRVLAWLDKLDEDRSFISIVSVAEIRRGVALMDEGRKRDALAEWLARDLPQRFEGRIIPMD
EPVALAWGDLMGPAKRNGRGLSSIDGLIAATAVAHDLTLATRNTRDFEGFGIELIDPWIA

Download         Length: 423 bp

>T45839 NZ_CP006991:661845-662267 [Rhizobium sp. IE4771]
GTGAGGCTTCTGCTGGACACCAACGTGCTGTTGGAGGTGACGAAGCCGAACCCCGAAATGCGCGTCTTGGCTTGGCTGGA
TAAGCTGGATGAGGATCGCTCCTTCATCAGCATCGTGTCGGTCGCGGAGATCCGGCGCGGTGTTGCCCTGATGGACGAGG
GACGAAAACGCGACGCGCTGGCCGAATGGCTTGCCCGGGACCTGCCGCAACGCTTCGAGGGCCGCATCATTCCGATGGAC
GAGCCGGTTGCCTTAGCCTGGGGTGACCTTATGGGCCCCGCGAAGCGGAACGGCCGCGGCCTGTCGTCGATAGATGGACT
GATCGCTGCAACCGCGGTTGCTCACGATCTCACTCTCGCCACGCGCAACACCAGGGATTTCGAGGGGTTCGGGATCGAAC
TCATCGATCCCTGGATCGCATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9804.01 Da        Isoelectric Point: 8.7841

>AT45839 WP_040143124.1 NZ_CP006991:661582-661848 [Rhizobium sp. IE4771]
MRPSRQGDESWTVASAKAKLSEVIERAQSAPQTIARNGKPSVVVVSAEEWQRKTARKGTLAEFLMSSPLRGADLDLERQR
DDPRDPEL

Download         Length: 267 bp

>AT45839 NZ_CP006991:661582-661848 [Rhizobium sp. IE4771]
ATGCGACCGTCACGGCAAGGCGATGAAAGCTGGACCGTCGCCAGCGCAAAGGCAAAGCTTTCCGAGGTTATCGAACGCGC
GCAATCCGCGCCGCAAACGATTGCCCGGAACGGCAAGCCGAGCGTCGTCGTCGTCTCCGCCGAGGAGTGGCAGCGCAAGA
CTGCGCGCAAGGGTACGCTTGCCGAATTCCTGATGAGTTCGCCGCTGCGTGGCGCCGATCTGGATCTCGAGCGTCAACGC
GACGATCCGCGTGATCCCGAACTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060IDY5

Antitoxin

Source ID Structure
AlphaFold DB A0A060IA78

References