Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 285406..286043 Replicon plasmid pRetIE4771e
Accession NZ_CP006991
Organism Rhizobium sp. IE4771

Toxin (Protein)


Gene name vapC Uniprot ID A0A060ICY2
Locus tag IE4771_RS31120 Protein ID WP_010059100.1
Coordinates 285642..286043 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name VapB1 Uniprot ID K0VQJ0
Locus tag IE4771_RS31115 Protein ID WP_008530479.1
Coordinates 285406..285642 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IE4771_RS31100 281400..282590 - 1191 WP_040112776.1 DUF1173 domain-containing protein -
IE4771_RS31105 282970..284007 + 1038 WP_040142816.1 toprim domain-containing protein -
IE4771_RS31110 284317..285255 + 939 WP_040112778.1 DUF2493 domain-containing protein -
IE4771_RS31115 285406..285642 + 237 WP_008530479.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
IE4771_RS31120 285642..286043 + 402 WP_010059100.1 type II toxin-antitoxin system VapC family toxin Toxin
IE4771_RS31125 286180..286548 - 369 WP_040112779.1 WGR domain-containing protein -
IE4771_RS31130 286972..287295 + 324 WP_008530485.1 DUF736 family protein -
IE4771_RS35250 287611..287778 + 168 WP_164519071.1 hypothetical protein -
IE4771_RS31135 288103..288585 + 483 WP_010054033.1 hypothetical protein -
IE4771_RS31140 288658..290577 - 1920 WP_040142819.1 Ti-type conjugative transfer system protein TraG -
IE4771_RS31145 290568..290807 - 240 WP_009988310.1 conjugal transfer protein TraD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..744104 744104


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-117)

Antitoxin

SpoVT-AbrB domain

(7-49)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14935.23 Da        Isoelectric Point: 5.1972

>T45838 WP_010059100.1 NZ_CP006991:285642-286043 [Rhizobium sp. IE4771]
MPLYMLDTNMCIYLMKEQPPQVARRFAQCRVGDVVMSAITFAELEYGVSASTNAEKERANLEFLIEAIPVAPFDANAARA
YGPVRFATRERKKDHLDKLIAAHALSIDAVLVTNNVRDFSVYEGLPIENWLLS

Download         Length: 402 bp

>T45838 NZ_CP006991:285642-286043 [Rhizobium sp. IE4771]
ATGCCGCTTTATATGCTCGACACCAATATGTGTATCTACTTGATGAAGGAGCAGCCGCCGCAAGTCGCGCGTCGCTTTGC
TCAATGCCGTGTTGGCGACGTCGTCATGTCAGCCATCACATTTGCGGAACTTGAATATGGTGTCTCCGCATCGACGAACG
CGGAAAAGGAGCGGGCAAACCTGGAATTCCTGATAGAAGCGATTCCAGTTGCGCCCTTCGACGCCAATGCGGCGCGGGCC
TACGGGCCTGTCAGGTTTGCCACGCGGGAACGGAAAAAAGATCATCTGGACAAATTGATAGCCGCTCATGCGCTCTCGAT
TGATGCTGTCCTCGTAACTAACAATGTGCGGGACTTTTCCGTCTATGAAGGGTTGCCGATCGAGAACTGGTTGTTGTCGT
GA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8940.06 Da        Isoelectric Point: 5.1299

>AT45838 WP_008530479.1 NZ_CP006991:285406-285642 [Rhizobium sp. IE4771]
MHTTKVFKNGNSQAVRIPADLAYSQTEMDLEIERIGDELRIRPLKPSLEGVLSRFARFDRSFMAEGRGDQEQAERDTL

Download         Length: 237 bp

>AT45838 NZ_CP006991:285406-285642 [Rhizobium sp. IE4771]
ATGCACACGACTAAGGTGTTCAAAAACGGCAACTCACAAGCTGTACGCATCCCCGCGGATTTGGCCTACAGCCAGACCGA
GATGGACCTTGAAATCGAGCGGATCGGTGACGAGCTTCGCATCCGCCCGCTAAAGCCGTCCCTGGAAGGCGTGCTGTCGC
GTTTCGCAAGGTTTGACCGCTCGTTTATGGCGGAGGGACGCGGCGATCAGGAGCAGGCCGAGCGGGATACGCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060ICY2

Antitoxin

Source ID Structure
AlphaFold DB K0VQJ0

References