Detailed information of TA system TA045837

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 260504..261171
• Replicon: plasmid pRetIE4771e
• Accession: NZ_CP006991
• Organism: Rhizobium sp. IE4771

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A060ICK5
• Locus tag: IE4771_RS30970
• Protein ID: WP_040142794.1
• Coordinates: 260504..260947 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: IE4771_RS30975
• Protein ID: WP_089152845.1
• Coordinates: 260944..261171 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IE4771_RS30940	257029..257418	+	390	WP_040112721.1	response regulator	-
IE4771_RS30945	257415..258662	+	1248	WP_040112722.1	response regulator	-
IE4771_RS30950	258659..259063	+	405	WP_009983201.1	hypothetical protein	-
IE4771_RS30955	259096..259326	+	231	WP_040112724.1	hypothetical protein	-
IE4771_RS30960	259591..259947	-	357	WP_010053142.1	type II toxin-antitoxin system HicB family antitoxin	-
IE4771_RS30965	259944..260198	-	255	WP_010053144.1	type II toxin-antitoxin system HicA family toxin	-
IE4771_RS30970	260504..260947	-	444	WP_040142794.1	PIN domain-containing protein	Toxin
IE4771_RS30975	260944..261171	-	228	WP_089152845.1	CopG family transcriptional regulator	Antitoxin
IE4771_RS30980	261567..261932	-	366	WP_010040844.1	GtrA family protein	-
IE4771_RS30985	261929..262633	-	705	WP_040112760.1	HAD family hydrolase	-
IE4771_RS30990	262630..263616	-	987	WP_040113156.1	UbiA prenyltransferase family protein	-
IE4771_RS30995	264213..264578	-	366	WP_040113157.1	hypothetical protein	-
IE4771_RS31000	264677..265216	+	540	WP_010041279.1	hypothetical protein	-
IE4771_RS31005	265220..265879	+	660	WP_040142796.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..744104	744104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-131)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 15559.89 Da
• Isoelectric Point: 8.1078

>T45837 WP_040142794.1 NZ_CP006991:c260947-260504 [Rhizobium sp. IE4771]
VTFLLDVNVLIALIDPGHVAHDDAHAWFAVVGQTAWATCPITENGVIRIVGNPKYPNSPGSPALVMEIVRKLRSLPGHSF
WPDDVSLVGSGDIAPTKILTPGQVTDTYLLALAKARGGQLATFDRKLSAAAVTRGNSALHLITTSRS

Nucleotide

• Length: 444 bp

>T45837 NZ_CP006991:c260947-260504 [Rhizobium sp. IE4771]
GTGACTTTCCTGCTCGACGTCAATGTGCTGATTGCCTTAATCGATCCGGGACATGTAGCCCATGATGATGCACACGCATG
GTTCGCAGTGGTGGGTCAGACCGCGTGGGCTACCTGCCCCATTACCGAAAATGGTGTCATCCGGATTGTCGGCAACCCCA
AATATCCCAACTCTCCCGGTTCACCGGCACTCGTGATGGAGATCGTCCGCAAATTGCGGTCGCTCCCCGGTCACAGCTTT
TGGCCGGACGATGTGAGCCTTGTCGGGTCAGGTGATATCGCGCCCACGAAAATTCTAACACCGGGACAGGTAACTGATAC
CTACCTGCTTGCGCTAGCCAAGGCGCGTGGTGGCCAGCTGGCGACATTCGATCGCAAGCTTTCAGCGGCAGCGGTTACAA
GGGGCAATTCTGCCTTGCACCTGATCACCACGAGTAGATCGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8210.47 Da
• Isoelectric Point: 7.0120

>AT45837 WP_089152845.1 NZ_CP006991:c261171-260944 [Rhizobium sp. IE4771]
MRTTLAIDDDVLIAAKAMATQQHRSVGEVISELARRSLRRPPSIGERNGIPLLSARPDTPPVTLEIVNALRDELP

Nucleotide

• Length: 228 bp

>AT45837 NZ_CP006991:c261171-260944 [Rhizobium sp. IE4771]
ATGAGAACGACTTTGGCAATCGACGACGATGTCCTGATCGCCGCAAAGGCGATGGCAACCCAGCAGCACCGGAGCGTCGG
CGAGGTCATTTCTGAGCTGGCGAGACGTTCTCTGCGCCGACCGCCCAGCATCGGTGAACGCAATGGCATTCCTCTCCTGT
CAGCTCGGCCGGATACGCCGCCAGTGACGCTCGAAATCGTGAACGCCTTGCGTGACGAACTGCCGTGA

Structures

Toxin

• AlphaFold DB: A0A060ICK5