Detailed information of TA system TA045836

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 22720..23534
• Replicon: plasmid pRetIE4771b
• Accession: NZ_CP006988
• Organism: Rhizobium sp. IE4771

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: A0A060I7A5
• Locus tag: IE4771_RS22755
• Protein ID: WP_040140266.1
• Coordinates: 23007..23534 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: -
• Locus tag: IE4771_RS22750
• Protein ID: WP_010047188.1
• Coordinates: 22720..23010 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IE4771_RS22725	18198..19736	+	1539	WP_040140264.1	GNAT family N-acetyltransferase	-
IE4771_RS22730	19733..20302	+	570	WP_051649913.1	AAA family ATPase	-
IE4771_RS34085	21437..21703	+	267	WP_080711058.1	hypothetical protein	-
IE4771_RS22745	21892..22638	+	747	WP_040140688.1	hypothetical protein	-
IE4771_RS22750	22720..23010	+	291	WP_010047188.1	DUF1778 domain-containing protein	Antitoxin
IE4771_RS22755	23007..23534	+	528	WP_040140266.1	hypothetical protein	Toxin
IE4771_RS22760	23661..25508	-	1848	WP_040140268.1	AAA family ATPase	-
IE4771_RS22765	25508..25996	-	489	WP_040140270.1	hypothetical protein	-
IE4771_RS22770	25984..27015	-	1032	WP_051649937.1	hypothetical protein	-
IE4771_RS22775	27191..27529	-	339	WP_186007926.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..393846	393846

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (9-90)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 18680.60 Da
• Isoelectric Point: 7.7528

>T45836 WP_040140266.1 NZ_CP006988:23007-23534 [Rhizobium sp. IE4771]
MTLPAWHEEPIAKSHGRAVFDCGEGQMNEFLLRFARQSHEQNASKTFCAIDDAVPGRILGFYTIAPSAVEHATVPDAMTR
GLARHDVPGFRLARLATDCAVAGQGLGGRLLAAAALRCLRLAGEGGGLLLIIDAKGERAANWYASYGAEPLKDKPLTLVM
PLITFAADLRAKGLL

Nucleotide

• Length: 528 bp

>T45836 NZ_CP006988:23007-23534 [Rhizobium sp. IE4771]
ATGACTTTACCTGCATGGCATGAGGAACCGATCGCTAAGTCTCACGGTCGGGCAGTCTTCGATTGCGGCGAGGGTCAGAT
GAACGAGTTCCTGCTCCGCTTTGCCCGACAGAGCCATGAACAGAATGCCTCAAAAACCTTCTGTGCTATCGACGATGCAG
TGCCCGGCCGTATTCTTGGGTTCTATACGATCGCGCCTTCGGCAGTCGAGCATGCGACTGTGCCTGATGCAATGACACGC
GGTCTTGCCCGCCATGATGTGCCGGGCTTCAGACTTGCTCGACTGGCTACCGACTGCGCGGTTGCCGGGCAGGGACTGGG
AGGCCGGCTTCTAGCCGCCGCAGCGCTCCGCTGCCTGCGACTTGCAGGAGAGGGCGGGGGGCTTCTACTGATCATCGATG
CCAAAGGCGAGCGAGCCGCAAACTGGTACGCTTCCTATGGTGCCGAACCGCTGAAGGACAAGCCGCTAACTCTCGTAATG
CCGCTCATCACCTTCGCGGCCGACTTGCGTGCCAAAGGTCTCCTTTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10397.99 Da
• Isoelectric Point: 7.0177

>AT45836 WP_010047188.1 NZ_CP006988:22720-23010 [Rhizobium sp. IE4771]
MEAEVPSIANENSERMSLRVPATAKAKLVRAAALRNTDLTDFVTQSALREAEAVIEEAETIKVSKRDHLRILELLENPPK
PNAKLRAAIAGLPGGL

Nucleotide

• Length: 291 bp

>AT45836 NZ_CP006988:22720-23010 [Rhizobium sp. IE4771]
ATGGAGGCCGAGGTGCCGAGCATAGCGAACGAGAACAGCGAGCGTATGAGTTTGAGGGTGCCTGCCACGGCCAAGGCTAA
GCTCGTGCGCGCTGCGGCACTTCGTAATACTGATCTGACGGATTTCGTAACGCAATCCGCGCTGCGAGAAGCAGAGGCTG
TGATCGAGGAAGCCGAGACGATCAAAGTATCGAAGCGCGATCATCTTCGCATTCTTGAGCTTCTGGAAAATCCGCCTAAA
CCTAACGCCAAGCTGCGCGCGGCGATTGCCGGTTTGCCTGGCGGCCTATGA

Structures

Toxin

• AlphaFold DB: A0A060I7A5