Detailed information of TA system TA045830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 943101..943660
• Replicon: chromosome
• Accession: NZ_CP006961
• Organism: Brucella suis bv.

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: D6LP74
• Locus tag: BSS2_RS04530
• Protein ID: WP_004688321.1
• Coordinates: 943415..943660 (-)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q57DF1
• Locus tag: BSS2_RS04525
• Protein ID: WP_002964097.1
• Coordinates: 943101..943415 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BSS2_RS04475	938791..938994	-	204	WP_004690812.1	hypothetical protein	-
BSS2_RS04480	938997..939212	-	216	WP_002971459.1	hypothetical protein	-
BSS2_RS04485	939209..939412	-	204	WP_002964088.1	hypothetical protein	-
BSS2_RS04490	939462..940181	+	720	WP_006072696.1	hypothetical protein	-
BSS2_RS04495	940178..940408	+	231	WP_002964091.1	hypothetical protein	-
BSS2_RS04500	940405..940680	+	276	WP_002964092.1	hypothetical protein	-
BSS2_RS04505	940703..941290	+	588	WP_004683738.1	hypothetical protein	-
BSS2_RS04510	941525..942235	+	711	WP_004683739.1	porin family protein	-
BSS2_RS04515	942583..942753	-	171	WP_002964095.1	DUF1508 domain-containing protein	-
BSS2_RS04525	943101..943415	-	315	WP_002964097.1	BrnA antitoxin family protein	Antitoxin
BSS2_RS04530	943415..943660	-	246	WP_004688321.1	BrnT family toxin	Toxin
BSS2_RS04535	943793..944431	+	639	Protein_890	tRNA-guanine transglycosylase	-
BSS2_RS17285	944501..945259	+	759	Protein_891	IS5 family transposase	-
BSS2_RS04545	945260..946033	+	774	WP_006132649.1	hypothetical protein	-
BSS2_RS04550	946168..947814	+	1647	WP_004689673.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	wboA / wbdA	937019..951130	14111
	-	flank	IS/Tn	-	-	944501..944869	368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-78)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-102)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9172.86 Da
• Isoelectric Point: 10.3158

>T45830 WP_004688321.1 NZ_CP006961:c943660-943415 [Brucella suis bv. 1 str. S2]
MKIIWDEPKRQTNIAKHGLDFADLHFEFFLSAKVFPTKADRLMAIGEFNGLIIIAVIFKPVGSEALSVISMRPASQKERK
L

Nucleotide

• Length: 246 bp

>T45830 NZ_CP006961:c943660-943415 [Brucella suis bv. 1 str. S2]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11638.42 Da
• Isoelectric Point: 10.3131

>AT45830 WP_002964097.1 NZ_CP006961:c943415-943101 [Brucella suis bv. 1 str. S2]
MGIKFSSKRPLTQEEEAEIQKMIASDPDAPEATDEQLAKAKPFKEAFPDMAAKMEKAIRGRPRIDNPKTPVTIRLDQDVV
QRFKATGKGWQGRMNDALRKAVGL

Nucleotide

• Length: 315 bp

>AT45830 NZ_CP006961:c943415-943101 [Brucella suis bv. 1 str. S2]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCCGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA