Detailed information of TA system TA045829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1966799..1967716
• Replicon: chromosome
• Accession: NZ_CP006960
• Organism: Bacillus amyloliquefaciens UMAF6614

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: BAMY6614_RS09875
• Protein ID: WP_007407256.1
• Coordinates: 1966970..1967716 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2C3Z8
• Locus tag: BAMY6614_RS09870
• Protein ID: WP_014417527.1
• Coordinates: 1966799..1966969 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BAMY6614_RS09835	1962032..1963654	+	1623	WP_061862105.1	pyocin knob domain-containing protein	-
BAMY6614_RS09840	1963667..1964038	+	372	WP_061862106.1	hypothetical protein	-
BAMY6614_RS09845	1964043..1964240	+	198	WP_007610833.1	XkdX family protein	-
BAMY6614_RS09850	1964297..1965058	+	762	WP_014417526.1	hypothetical protein	-
BAMY6614_RS09855	1965110..1965373	+	264	WP_003154815.1	hemolysin XhlA family protein	-
BAMY6614_RS09860	1965387..1965650	+	264	WP_003154813.1	phage holin	-
BAMY6614_RS09865	1965664..1966542	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
BAMY6614_RS09870	1966799..1966969	-	171	WP_014417527.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
BAMY6614_RS09875	1966970..1967716	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BAMY6614_RS09880	1967820..1968818	-	999	WP_003154805.1	inorganic phosphate transporter	-
BAMY6614_RS09885	1968831..1969448	-	618	WP_003154804.1	DUF47 domain-containing protein	-
BAMY6614_RS09890	1969734..1971050	-	1317	WP_061862107.1	amino acid permease	-
BAMY6614_RS09895	1971373..1972323	+	951	WP_007610844.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1933169..1976009	42840

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T45829 WP_007407256.1 NZ_CP006960:c1967716-1966970 [Bacillus amyloliquefaciens UMAF6614]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T45829 NZ_CP006960:c1967716-1966970 [Bacillus amyloliquefaciens UMAF6614]
ATGCTTTTGTTTTTTCAGATTATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.78 Da
• Isoelectric Point: 10.9951

>AT45829 WP_014417527.1 NZ_CP006960:c1966969-1966799 [Bacillus amyloliquefaciens UMAF6614]
MEQAFQNGQPEKRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT45829 NZ_CP006960:c1966969-1966799 [Bacillus amyloliquefaciens UMAF6614]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAAAAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2C3Z8