Detailed information of TA system TA045828

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1206732..1207369
• Replicon: chromosome
• Accession: NZ_CP006960
• Organism: Bacillus amyloliquefaciens UMAF6614

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: BAMY6614_RS06005
• Protein ID: WP_003156187.1
• Coordinates: 1207019..1207369 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: BAMY6614_RS06000
• Protein ID: WP_003156188.1
• Coordinates: 1206732..1207013 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BAMY6614_RS05980	1203097..1203696	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
BAMY6614_RS05985	1203789..1204154	+	366	WP_061861876.1	holo-ACP synthase	-
BAMY6614_RS05990	1204319..1205326	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
BAMY6614_RS05995	1205443..1206612	+	1170	WP_053285486.1	alanine racemase	-
BAMY6614_RS06000	1206732..1207013	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
BAMY6614_RS06005	1207019..1207369	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BAMY6614_RS06010	1207487..1208308	+	822	WP_003156182.1	STAS domain-containing protein	-
BAMY6614_RS06015	1208313..1208678	+	366	WP_007609584.1	RsbT antagonist protein RsbS	-
BAMY6614_RS06020	1208681..1209082	+	402	WP_003156178.1	anti-sigma regulatory factor	-
BAMY6614_RS06025	1209094..1210101	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
BAMY6614_RS06030	1210165..1210494	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
BAMY6614_RS06035	1210491..1210973	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
BAMY6614_RS06040	1210939..1211727	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
BAMY6614_RS06045	1211727..1212329	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T45828 WP_003156187.1 NZ_CP006960:1207019-1207369 [Bacillus amyloliquefaciens UMAF6614]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T45828 NZ_CP006960:1207019-1207369 [Bacillus amyloliquefaciens UMAF6614]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT45828 WP_003156188.1 NZ_CP006960:1206732-1207013 [Bacillus amyloliquefaciens UMAF6614]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT45828 NZ_CP006960:1206732-1207013 [Bacillus amyloliquefaciens UMAF6614]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5