Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-MazE
Location 2049345..2049995 Replicon chromosome
Accession NZ_CP006957
Organism Mannheimia haemolytica USDA-ARS-USMARC-184

Toxin (Protein)


Gene name vapC Uniprot ID A0A547EDJ6
Locus tag B824_RS10285 Protein ID WP_006249411.1
Coordinates 2049345..2049734 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1D2Q6P1
Locus tag B824_RS10290 Protein ID WP_006251766.1
Coordinates 2049735..2049995 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B824_RS10235 2044700..2045509 - 810 WP_006250123.1 metal ABC transporter permease -
B824_RS10240 2045502..2046362 - 861 WP_006250124.1 metal ABC transporter permease -
B824_RS10245 2046436..2047221 - 786 WP_006251768.1 hypothetical protein -
B824_RS10280 2048087..2049325 - 1239 WP_006251767.1 peptidase T -
B824_RS10285 2049345..2049734 - 390 WP_006249411.1 type II toxin-antitoxin system VapC family toxin Toxin
B824_RS10290 2049735..2049995 - 261 WP_006251766.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
B824_RS10295 2050111..2050323 - 213 WP_006251765.1 BrnA antitoxin family protein -
B824_RS10300 2050324..2050611 - 288 WP_006251764.1 BrnT family toxin -
B824_RS10305 2050774..2054307 - 3534 WP_040081125.1 transcription-repair coupling factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-119)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 14633.91 Da        Isoelectric Point: 7.1041

>T45827 WP_006249411.1 NZ_CP006957:c2049734-2049345 [Mannheimia haemolytica USDA-ARS-USMARC-184]
MYMLDTNTVSYFFRGNANVVAKLKSINPERLCISSVTAAELVYGVAKRNNGQLSQFLDYFLSSVKVLDWNYRCAELYGKL
RAEMEKNGKVMGVQDQMIASHALAEECILVSSDQAFKMVPDLKLENWLH

Download         Length: 390 bp

>T45827 NZ_CP006957:c2049734-2049345 [Mannheimia haemolytica USDA-ARS-USMARC-184]
ATGTATATGTTAGATACGAATACTGTAAGCTATTTTTTTCGTGGAAATGCAAATGTGGTGGCGAAATTGAAGTCCATTAA
TCCTGAGAGGCTTTGTATTTCATCTGTAACTGCTGCTGAATTGGTTTATGGTGTGGCGAAAAGAAATAATGGGCAGCTTT
CTCAATTTTTAGATTATTTTTTATCGAGTGTAAAGGTTTTAGATTGGAATTATCGTTGTGCTGAACTTTATGGAAAGTTA
CGGGCAGAGATGGAGAAAAATGGCAAAGTAATGGGCGTGCAGGATCAAATGATTGCTAGTCACGCTCTTGCTGAAGAGTG
TATTTTAGTTTCTAGCGATCAGGCATTTAAAATGGTACCTGATCTGAAATTGGAGAATTGGTTACACTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 10245.50 Da        Isoelectric Point: 4.6909

>AT45827 WP_006251766.1 NZ_CP006957:c2049995-2049735 [Mannheimia haemolytica USDA-ARS-USMARC-184]
MEKIARLFRNGRNQAVRLPVEFEFDAEQVYVSKQENGDILLSLRSRNTEHWQRLFELLPAVVCDETFLDTKERNQSFEQR
DPFEVA

Download         Length: 261 bp

>AT45827 NZ_CP006957:c2049995-2049735 [Mannheimia haemolytica USDA-ARS-USMARC-184]
ATGGAAAAAATTGCGAGATTATTCCGTAACGGGCGGAACCAAGCAGTGCGTTTACCGGTTGAATTTGAGTTTGATGCAGA
GCAAGTCTATGTTTCTAAACAAGAAAATGGCGATATTTTATTATCATTGAGATCGAGAAATACCGAGCATTGGCAACGAT
TGTTTGAGCTATTGCCTGCTGTTGTATGTGATGAAACATTTTTAGATACAAAAGAGAGAAACCAATCCTTTGAGCAACGG
GATCCTTTTGAGGTAGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A547EDJ6

Antitoxin

Source ID Structure
AlphaFold DB A0A1D2Q6P1

References