Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /BrnT(toxin)
Location 735806..736328 Replicon chromosome
Accession NZ_CP006957
Organism Mannheimia haemolytica USDA-ARS-USMARC-184

Toxin (Protein)


Gene name - Uniprot ID A0A249A107
Locus tag B824_RS03830 Protein ID WP_006249269.1
Coordinates 735806..736102 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A249A190
Locus tag B824_RS03835 Protein ID WP_006249270.1
Coordinates 736086..736328 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B824_RS03815 730860..732185 + 1326 WP_020831251.1 deoxyguanosinetriphosphate triphosphohydrolase family protein -
B824_RS03820 732306..732605 + 300 WP_006249265.1 hypothetical protein -
B824_RS03825 732767..735679 + 2913 WP_006249267.1 RNA polymerase-associated protein RapA -
B824_RS03830 735806..736102 + 297 WP_006249269.1 BrnT family toxin Toxin
B824_RS03835 736086..736328 + 243 WP_006249270.1 CopG family transcriptional regulator Antitoxin
B824_RS03840 736340..737008 + 669 WP_006249271.1 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA -
B824_RS03845 736986..737561 - 576 WP_006249272.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
B824_RS03850 737621..738463 + 843 WP_006250575.1 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ -
B824_RS03855 738586..739227 + 642 WP_040080687.1 orotate phosphoribosyltransferase -
B824_RS03860 739401..740478 + 1078 Protein_698 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(15-90)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 12009.89 Da        Isoelectric Point: 7.9454

>T45825 WP_006249269.1 NZ_CP006957:735806-736102 [Mannheimia haemolytica USDA-ARS-USMARC-184]
MYIDLQYAVPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKVVLEACIEPELRYFLIGKIYDKYWTAFFTPRNDVIRL
ISVRRSRKKEISYYEKYH

Download         Length: 297 bp

>T45825 NZ_CP006957:735806-736102 [Mannheimia haemolytica USDA-ARS-USMARC-184]
ATGTATATAGATTTGCAATATGCTGTGCCGTTATTGTTTGAATACGACCCGAACAAAAGCCAAATTAATTTAGCCAAATA
TGGTATTGATTTTGAACAGGCAAAGTTGCTTTGGGAAGATGAACGAAAAGTTGTACTGGAGGCTTGCATTGAACCCGAAT
TACGCTATTTCCTGATTGGCAAGATTTATGATAAATATTGGACAGCGTTTTTTACACCAAGAAATGATGTTATTCGTTTA
ATTTCAGTTAGACGTTCCCGTAAAAAAGAGATCTCATACTATGAGAAATACCATTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9346.79 Da        Isoelectric Point: 7.1665

>AT45825 WP_006249270.1 NZ_CP006957:736086-736328 [Mannheimia haemolytica USDA-ARS-USMARC-184]
MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFPNWMVEALDKQARHLGVPRQALIKMWIAEKLPVQSPR

Download         Length: 243 bp

>AT45825 NZ_CP006957:736086-736328 [Mannheimia haemolytica USDA-ARS-USMARC-184]
ATGAGAAATACCATTAGTGCCGAAGAATTAGAACGTATCTTCGATGAAGGCAAAGAAGATATTTTACAATACTGCGATCT
TTCTACCGCTCATCGCCCGGGGCAAATGAAAAGGGTGAATGTTGATTTCCCAAATTGGATGGTAGAAGCGTTAGATAAGC
AAGCCAGACATTTAGGTGTGCCTCGCCAAGCCTTGATTAAAATGTGGATTGCGGAAAAATTACCGGTTCAATCACCTAGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A249A107

Antitoxin

Source ID Structure
AlphaFold DB A0A249A190

References