Detailed information of TA system TA045824

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 309625..310147
• Replicon: chromosome
• Accession: NZ_CP006957
• Organism: Mannheimia haemolytica USDA-ARS-USMARC-184

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: B824_RS01600
• Protein ID: WP_040080572.1
• Coordinates: 309625..309894 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: A0A248ZY54
• Locus tag: B824_RS01605
• Protein ID: WP_006250323.1
• Coordinates: 309881..310147 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B824_RS01580	304945..305379	-	435	WP_006250318.1	D-tyrosyl-tRNA(Tyr) deacylase	-
B824_RS01585	305354..306199	-	846	WP_006251985.1	virulence factor BrkB family protein	-
B824_RS01590	306201..307439	-	1239	WP_006251984.1	glutamate-5-semialdehyde dehydrogenase	-
B824_RS01595	307723..309606	+	1884	WP_040080571.1	DNA mismatch repair endonuclease MutL	-
B824_RS01600	309625..309894	-	270	WP_040080572.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
B824_RS01605	309881..310147	-	267	WP_006250323.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
B824_RS01610	310587..311540	+	954	WP_040080573.1	tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA	-
B824_RS01615	311609..311881	+	273	WP_006250326.1	RNA chaperone Hfq	-
B824_RS01620	311901..313286	+	1386	WP_006251981.1	GTPase HflX	-
B824_RS01630	313624..314664	+	1041	WP_040080511.1	IS481 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-84)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10508.09 Da
• Isoelectric Point: 6.4732

>T45824 WP_040080572.1 NZ_CP006957:c309894-309625 [Mannheimia haemolytica USDA-ARS-USMARC-184]
MREIEYSNAFKRDYKKCAVEFLSSSLIEALYFLINDKPLPEKYCDHALTGEWKGFRDCHIQPDLVLIYRKVEDDKLELLR
IGSHSHLFG

Nucleotide

• Length: 270 bp

>T45824 NZ_CP006957:c309894-309625 [Mannheimia haemolytica USDA-ARS-USMARC-184]
ATGCGAGAGATTGAGTATAGTAATGCTTTTAAACGTGACTATAAAAAATGTGCGGTTGAGTTTTTATCCTCTTCATTAAT
TGAGGCGTTGTATTTTCTGATTAATGACAAGCCATTACCTGAGAAATACTGCGATCACGCTTTAACCGGCGAATGGAAAG
GCTTTAGGGATTGCCATATCCAACCTGATTTGGTTCTTATCTATCGAAAAGTGGAAGATGATAAGCTAGAGCTGCTGCGA
ATTGGCAGCCACTCTCATTTATTTGGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9738.10 Da
• Isoelectric Point: 4.7954

>AT45824 WP_006250323.1 NZ_CP006957:c310147-309881 [Mannheimia haemolytica USDA-ARS-USMARC-184]
MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATEKRFQFDYQPNFETAEVLRAVKNGEEKLHSANSIADL
MEQLNARD

Nucleotide

• Length: 267 bp

>AT45824 NZ_CP006957:c310147-309881 [Mannheimia haemolytica USDA-ARS-USMARC-184]
ATGGCAAATAGTGCATTAGTAAGCGTAAGAATCAGCCCTGAAATTAAAGAGCAAGCAAGTGAGGTGTTAGCCGGTATCGG
CTTGACTGTCTCTGATGTGATGCGAATGACATTAGCGAAAATCGCAACAGAAAAACGTTTTCAGTTTGATTATCAGCCTA
ATTTTGAGACAGCAGAAGTATTAAGGGCTGTTAAGAATGGCGAGGAAAAGCTACATTCAGCAAATAGTATTGCGGATTTA
ATGGAGCAACTCAATGCGAGAGATTGA

Structures

Antitoxin

• AlphaFold DB: A0A248ZY54