Detailed information of TA system TA045823

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 49622..50337
• Replicon: chromosome
• Accession: NZ_CP006957
• Organism: Mannheimia haemolytica USDA-ARS-USMARC-184

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: B824_RS00215
• Protein ID: WP_006250491.1
• Coordinates: 49622..50050 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A248ZW87
• Locus tag: B824_RS00220
• Protein ID: WP_006248272.1
• Coordinates: 50050..50337 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B824_RS00200	44877..46529	+	1653	WP_040080487.1	phospho-sugar mutase	-
B824_RS00205	46582..47515	+	934	Protein_41	nucleoside hydrolase	-
B824_RS00210	47537..49618	-	2082	WP_040080489.1	ATP-dependent DNA helicase RecG	-
B824_RS00215	49622..50050	-	429	WP_006250491.1	type II toxin-antitoxin system VapC family toxin	Toxin
B824_RS00220	50050..50337	-	288	WP_006248272.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
B824_RS00225	50501..52384	+	1884	WP_040080490.1	molecular chaperone HtpG	-
B824_RS00230	52437..52619	+	183	WP_006250493.1	hypothetical protein	-
B824_RS00240	52764..53804	+	1041	WP_040080492.1	IS481 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	52764..53804	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-129)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 16640.06 Da
• Isoelectric Point: 8.9269

>T45823 WP_006250491.1 NZ_CP006957:c50050-49622 [Mannheimia haemolytica USDA-ARS-USMARC-184]
MYRYLFDTNIVSELYKLRNDKMDVNVRQWLRGVNPSQTNISCITLSEIKTGILLKARKDKEQSDRLNDWFENNVLKAYQT
KAFVVNNDIALLASEYHIPNKMDLNDAYIAATAKYHNLILVTRNTKDFIRCDVRLFNPFETA

Nucleotide

• Length: 429 bp

>T45823 NZ_CP006957:c50050-49622 [Mannheimia haemolytica USDA-ARS-USMARC-184]
ATGTATCGCTACCTTTTTGATACCAATATAGTTTCCGAGCTTTATAAGTTAAGAAATGACAAGATGGATGTCAATGTTCG
CCAGTGGCTGAGAGGGGTTAATCCTAGCCAGACCAATATCAGCTGTATTACGTTATCTGAAATCAAAACAGGTATTTTGC
TGAAAGCCCGCAAAGACAAAGAACAATCAGACCGATTAAATGATTGGTTTGAAAATAATGTATTAAAGGCTTATCAAACA
AAAGCGTTTGTGGTGAATAACGACATTGCTCTATTGGCATCAGAGTACCATATTCCCAATAAAATGGATTTAAATGATGC
TTATATTGCGGCAACCGCCAAATACCACAATCTGATTTTAGTCACCCGCAACACCAAAGATTTTATCCGTTGTGATGTGC
GTTTATTTAACCCATTTGAAACCGCATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11085.39 Da
• Isoelectric Point: 4.3648

>AT45823 WP_006248272.1 NZ_CP006957:c50337-50050 [Mannheimia haemolytica USDA-ARS-USMARC-184]
MAITITSREFNQNASKVLKMAEKEPVFITKWGKIVNVVSSYMDYQQKKPSEPSFEELFASSENEDVSDEFWQEFEQTLVE
IRKSNKPRPVDFGDE

Nucleotide

• Length: 288 bp

>AT45823 NZ_CP006957:c50337-50050 [Mannheimia haemolytica USDA-ARS-USMARC-184]
ATGGCAATAACCATTACTTCCAGAGAATTTAATCAAAATGCCAGCAAAGTGTTAAAAATGGCAGAAAAAGAGCCGGTTTT
TATTACCAAATGGGGCAAAATCGTCAATGTGGTGTCTAGTTATATGGATTATCAGCAGAAAAAGCCTAGCGAGCCAAGCT
TTGAAGAGCTATTCGCCTCTTCCGAAAATGAGGATGTGAGCGATGAATTTTGGCAAGAGTTTGAACAAACATTGGTTGAA
ATCCGCAAAAGCAATAAACCAAGACCTGTTGATTTTGGAGATGAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A248ZW87