Detailed information of TA system TA045822

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 641544..643078
• Replicon: chromosome
• Accession: NZ_CP006955
• Organism: Bibersteinia trehalosi USDA-ARS-USMARC-189

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A4V7I8D4
• Locus tag: F543_RS03290
• Protein ID: WP_015433059.1
• Coordinates: 641792..643078 (+)
• Length: 429 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: F543_RS03285
• Protein ID: WP_015433060.1
• Coordinates: 641544..641798 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F543_RS03255	636649..637290	+	642	WP_015433067.1	orotate phosphoribosyltransferase	-
F543_RS03260	637341..637922	-	582	WP_015433066.1	rhomboid family intramembrane serine protease	-
F543_RS03265	637915..638544	-	630	WP_015433065.1	DsbA family oxidoreductase	-
F543_RS03270	638548..639000	-	453	WP_025266848.1	hypothetical protein	-
F543_RS03275	639234..640493	-	1260	WP_038445701.1	MFS transporter	-
F543_RS03280	640702..641106	+	405	Protein_646	ABC transporter ATP-binding protein	-
F543_RS11725	641243..641428	+	186	Protein_647	ATP-binding protein	-
F543_RS03285	641544..641798	+	255	WP_015433060.1	helix-turn-helix domain-containing protein	Antitoxin
F543_RS03290	641792..643078	+	1287	WP_015433059.1	type II toxin-antitoxin system HipA family toxin	Toxin
F543_RS03295	643150..643578	-	429	WP_015433058.1	helix-turn-helix domain-containing protein	-
F543_RS03300	643727..644728	+	1002	WP_015433057.1	TerC/Alx family metal homeostasis membrane protein	-
F543_RS03305	644785..645507	+	723	WP_155807099.1	hypothetical protein	-
F543_RS03310	645547..646122	+	576	WP_015433055.1	TerD family protein	-
F543_RS03315	646170..646844	+	675	WP_038445703.1	stress protein	-
F543_RS03320	646910..648058	-	1149	WP_015433053.1	TIR domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	621675..648058	26383

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (148-394)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-64)

Sequences

Toxin

Protein

• Length: 429 a.a.
• Molecular weight: 48546.92 Da
• Isoelectric Point: 7.9839

>T45822 WP_015433059.1 NZ_CP006955:641792-643078 [Bibersteinia trehalosi USDA-ARS-USMARC-189]
MVGNVLTVAMNGLEVGHWQKLSSGATEFCYIPSWLNSPRKRAISLSLPLSDQIYRGDVVYNFFDNLLPDNELIRSRIQQR
FQTTTKSPFDLLSAIGQDCVGAIQLYNQTPSPTQNINAQKLVDQEIEHILQNYQSLPLGMAEQADFRISIAGAQEKTAFL
YHQGEWKRPLGSTPTTHIFKLPIGVVGQGQIDLSNSCENEWLCLNILRVFGLPVSQAEIAYFGETKVLIIERFDRRWAKD
NSYLLRLPQEDMCQAFNIAPARKYENDGGVGILPIMQLLKGSSNALADQKAFFKAQILFWLLCAIDGHAKNFSLFLEPNN
RYKLTPFYDVISAYPLISPKGLQKQKVKMAMAWHGEQNKHYLWDKIQLRHIFNTAKLAGLSKQTVEEILQEIVVASSRLN
SIPTQHLPEQIVDPILSEMERKLVVVNR

Nucleotide

• Length: 1287 bp

>T45822 NZ_CP006955:641792-643078 [Bibersteinia trehalosi USDA-ARS-USMARC-189]
ATGGTAGGCAATGTACTGACGGTTGCGATGAATGGGTTAGAAGTTGGGCATTGGCAAAAACTTTCTAGCGGAGCAACGGA
ATTTTGTTATATCCCGAGCTGGCTCAACTCTCCACGAAAACGAGCGATTTCTCTTTCATTACCGCTATCGGATCAAATTT
ATCGTGGTGATGTAGTCTATAATTTTTTCGATAATTTGTTGCCGGACAATGAATTAATTCGCTCCCGTATTCAGCAACGT
TTTCAAACCACCACAAAATCGCCGTTCGATTTGCTTTCGGCAATCGGGCAAGACTGTGTGGGAGCTATTCAGCTTTATAA
TCAAACACCTTCGCCCACTCAGAACATTAACGCTCAAAAGCTGGTAGATCAGGAAATTGAGCATATTTTACAAAATTATC
AAAGCCTGCCACTGGGTATGGCGGAGCAGGCAGATTTCCGTATTTCAATTGCTGGTGCGCAGGAAAAAACGGCCTTTCTT
TACCATCAAGGCGAATGGAAACGCCCGTTGGGTTCAACGCCAACCACACATATTTTCAAATTACCGATCGGTGTTGTTGG
ACAAGGGCAAATCGACCTTTCGAATAGTTGTGAAAATGAGTGGCTCTGTTTGAATATTTTGCGGGTATTTGGCTTGCCTG
TATCTCAGGCGGAAATTGCCTATTTTGGTGAAACCAAAGTGCTGATTATCGAACGTTTTGACCGCCGTTGGGCGAAGGAC
AATAGCTATTTGCTCCGTTTACCACAAGAAGATATGTGCCAAGCGTTTAACATTGCTCCGGCTCGGAAATATGAAAATGA
TGGTGGCGTTGGTATTCTGCCAATTATGCAACTCCTCAAAGGTTCGAGCAATGCACTTGCCGATCAAAAAGCCTTTTTCA
AAGCCCAAATTCTGTTTTGGCTGCTCTGTGCAATTGACGGACACGCCAAAAATTTCAGCCTATTTTTAGAACCTAACAAC
CGCTATAAACTTACGCCGTTTTACGATGTGATTTCCGCCTACCCATTGATTAGCCCGAAAGGCTTGCAAAAACAGAAAGT
TAAAATGGCAATGGCGTGGCACGGCGAGCAGAATAAGCACTATCTTTGGGATAAAATCCAACTTCGACATATTTTTAATA
CTGCAAAGTTAGCAGGCTTATCAAAACAGACAGTGGAGGAAATATTACAGGAGATTGTTGTGGCTTCATCACGATTAAAT
AGTATCCCGACACAACATTTGCCCGAACAAATTGTTGACCCGATTTTGAGTGAGATGGAGAGAAAGTTGGTTGTGGTGAA
TAGGTAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9528.92 Da
• Isoelectric Point: 4.7661

>AT45822 WP_015433060.1 NZ_CP006955:641544-641798 [Bibersteinia trehalosi USDA-ARS-USMARC-189]
MVATTPQMLAHAVKEYRNLQKKSQTEVATVANTKQSTISIFENDPTGTKIETLFNILMALELELVVQPRPKSRDILQDEE
GEIW

Nucleotide

• Length: 255 bp

>AT45822 NZ_CP006955:641544-641798 [Bibersteinia trehalosi USDA-ARS-USMARC-189]
ATGGTTGCTACCACGCCACAAATGTTGGCACACGCAGTGAAAGAATACCGTAACTTACAGAAAAAATCGCAAACTGAAGT
TGCCACAGTTGCTAACACTAAGCAATCGACGATCTCCATATTTGAAAATGATCCCACTGGCACAAAAATTGAAACTTTGT
TCAATATTTTAATGGCATTAGAATTAGAGTTAGTCGTCCAGCCTCGCCCGAAAAGTCGTGATATTTTGCAAGATGAAGAG
GGCGAGATATGGTAG

Structures

Toxin

• AlphaFold DB: A0A4V7I8D4