Detailed information of TA system TA045799

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-YefM
• Location: 1956138..1956826
• Replicon: chromosome
• Accession: NZ_CP006943
• Organism: Mannheimia varigena USDA-ARS-USMARC-1296

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: W0QGL1
• Locus tag: X808_RS09315
• Protein ID: WP_025218061.1
• Coordinates: 1956404..1956826 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: W0QDM7
• Locus tag: X808_RS09310
• Protein ID: WP_025218060.1
• Coordinates: 1956138..1956404 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
X808_RS09290	1951569..1952408	-	840	WP_025218056.1	PhzF family phenazine biosynthesis protein	-
X808_RS09295	1952657..1953991	+	1335	WP_025218057.1	anaerobic C4-dicarboxylate transporter	-
X808_RS09300	1954355..1955014	-	660	WP_025218058.1	NAD(P)H-binding protein	-
X808_RS09305	1955062..1955910	-	849	WP_025218059.1	MBL fold metallo-hydrolase	-
X808_RS09310	1956138..1956404	+	267	WP_025218060.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
X808_RS09315	1956404..1956826	+	423	WP_025218061.1	type II toxin-antitoxin system VapC family toxin	Toxin
X808_RS09320	1956826..1957716	+	891	WP_025218062.1	LysR family transcriptional regulator	-
X808_RS09325	1957845..1958903	-	1059	WP_025218063.1	NAD(P)-dependent alcohol dehydrogenase	-
X808_RS09330	1958923..1959756	-	834	WP_025218064.1	S-formylglutathione hydrolase	-
X808_RS09335	1959828..1960937	-	1110	WP_025218065.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-61)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15921.47 Da
• Isoelectric Point: 7.5204

>T45799 WP_025218061.1 NZ_CP006943:1956404-1956826 [Mannheimia varigena USDA-ARS-USMARC-1296]
MFLLDTNVVSELRKVKKGMANPNVVAWLAKQNPNDFYINSIVLMELERGVLAMERKDPTQGIHLRHWQNLFLNQILHKPI
LPIDETTAQICAKLHIPDHAPENDAWIAASAIQHHLILVTRNTADFAKTGAKLFNPFEPQ

Nucleotide

• Length: 423 bp

>T45799 NZ_CP006943:1956404-1956826 [Mannheimia varigena USDA-ARS-USMARC-1296]
ATGTTTTTACTCGATACTAATGTGGTCAGCGAACTGCGAAAAGTGAAAAAAGGGATGGCTAATCCAAATGTAGTAGCGTG
GTTGGCAAAGCAAAATCCGAATGATTTTTACATTAATTCTATTGTGCTAATGGAACTGGAACGGGGCGTTTTGGCGATGG
AGCGAAAAGATCCTACGCAAGGTATTCATCTACGCCATTGGCAAAATCTGTTTCTCAACCAAATTTTGCATAAGCCGATT
TTACCCATTGACGAAACCACCGCCCAAATCTGTGCCAAACTGCATATTCCCGATCACGCCCCTGAAAATGATGCGTGGAT
TGCAGCCTCCGCTATTCAACACCATTTGATTTTGGTTACTCGCAATACAGCTGATTTCGCCAAAACAGGGGCAAAATTGT
TTAACCCCTTTGAGCCACAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10002.37 Da
• Isoelectric Point: 6.4594

>AT45799 WP_025218060.1 NZ_CP006943:1956138-1956404 [Mannheimia varigena USDA-ARS-USMARC-1296]
MSVMTCREFNQKTSLAQKYAETAPVIITHRGKPTFVLLKYEDYQPQKTARSALDALLDLDHSPDVANIELELKPRSRAQR
PPVDFGDE

Nucleotide

• Length: 267 bp

>AT45799 NZ_CP006943:1956138-1956404 [Mannheimia varigena USDA-ARS-USMARC-1296]
ATGAGTGTAATGACCTGCCGTGAATTTAATCAAAAAACCAGCCTTGCCCAAAAATATGCGGAGACCGCACCGGTGATTAT
TACCCATCGAGGTAAGCCGACTTTTGTGTTGTTGAAGTATGAGGATTATCAACCGCAAAAAACCGCTCGAAGTGCCTTAG
ACGCTTTGTTAGATCTGGATCATTCCCCCGATGTGGCGAATATTGAGTTGGAGTTAAAGCCCCGTAGTCGAGCCCAACGT
CCGCCGGTCGATTTTGGAGATGAGTGA

Structures

Toxin

• AlphaFold DB: W0QGL1

Antitoxin

• AlphaFold DB: W0QDM7