Detailed information of TA system TA045797

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 585889..586486
• Replicon: chromosome
• Accession: NZ_CP006943
• Organism: Mannheimia varigena USDA-ARS-USMARC-1296

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: W0Q9U1
• Locus tag: X808_RS02790
• Protein ID: WP_025216818.1
• Coordinates: 585889..586164 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: W0QCV8
• Locus tag: X808_RS02795
• Protein ID: WP_025216819.1
• Coordinates: 586157..586486 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
X808_RS02770	581705..582628	-	924	WP_025216814.1	oligopeptide ABC transporter permease OppB	-
X808_RS02775	582842..583609	-	768	WP_038643013.1	spermidine/putrescine ABC transporter permease PotC	-
X808_RS02780	583609..584472	-	864	WP_025216816.1	spermidine/putrescine ABC transporter permease PotB	-
X808_RS02785	584459..585574	-	1116	WP_025216817.1	spermidine/putrescine ABC transporter ATP-binding protein PotA	-
X808_RS02790	585889..586164	+	276	WP_025216818.1	type II toxin-antitoxin system HicA family toxin	Toxin
X808_RS02795	586157..586486	+	330	WP_025216819.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
X808_RS02800	586593..588611	+	2019	WP_025216820.1	DNA helicase Rep	-
X808_RS02805	588658..590112	-	1455	WP_025216821.1	aminoacyl-histidine dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (28-80)

Antitoxin

• Uncharacterised protein family HicB (56-105)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10260.96 Da
• Isoelectric Point: 9.5963

>T45797 WP_025216818.1 NZ_CP006943:585889-586164 [Mannheimia varigena USDA-ARS-USMARC-1296]
MVLIHLKRKHQITLSQIYTKPISANIKWKDIEALFLELGAEIEEREGSQIAVVLFGQVKVFHRPHPSPDTDKGAVASVKK
WLSENGVIPYA

Nucleotide

• Length: 276 bp

>T45797 NZ_CP006943:585889-586164 [Mannheimia varigena USDA-ARS-USMARC-1296]
ATGGTATTAATTCATCTTAAGCGAAAGCACCAAATCACACTTAGTCAAATTTATACCAAGCCGATTTCGGCGAATATCAA
ATGGAAAGATATTGAAGCTCTTTTTCTGGAATTAGGGGCTGAAATTGAAGAGCGAGAAGGAAGTCAGATTGCTGTGGTGT
TATTTGGTCAAGTAAAAGTGTTTCATAGACCGCACCCAAGCCCTGACACCGATAAAGGGGCGGTGGCAAGCGTGAAAAAA
TGGTTATCTGAAAACGGAGTAATTCCCTATGCGTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12310.01 Da
• Isoelectric Point: 4.5915

>AT45797 WP_025216819.1 NZ_CP006943:586157-586486 [Mannheimia varigena USDA-ARS-USMARC-1296]
MRNIMEINGYKAVIAYDPETELFRGEFLGLNGGADFYADNVAQLKQEGELSLKIFFEMCEEKGIEPFKQYSGKFNIRLSP
ELHKAAALAATAENLSLNEWISRTLEKSV

Nucleotide

• Length: 330 bp

>AT45797 NZ_CP006943:586157-586486 [Mannheimia varigena USDA-ARS-USMARC-1296]
ATGCGTAATATTATGGAGATTAATGGCTACAAAGCCGTGATTGCTTACGATCCTGAAACTGAACTCTTTCGGGGAGAATT
TCTCGGCTTAAATGGCGGAGCAGATTTCTACGCAGACAATGTGGCTCAATTAAAGCAAGAAGGCGAGCTAAGCCTAAAAA
TCTTTTTTGAAATGTGTGAAGAAAAAGGAATTGAGCCTTTCAAACAATATTCGGGCAAATTTAATATCCGCCTTTCACCG
GAATTACACAAAGCTGCAGCACTAGCCGCCACTGCAGAAAACCTCAGTTTAAATGAATGGATTAGCCGAACTTTGGAAAA
AAGTGTGTAA

Structures

Toxin

• AlphaFold DB: W0Q9U1

Antitoxin

• AlphaFold DB: W0QCV8