Detailed information of TA system TA045794

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 2132506..2133026
• Replicon: chromosome
• Accession: NZ_CP006942
• Organism: Mannheimia sp. USDA-ARS-USMARC-1261

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: X781_RS12105
• Protein ID: WP_081732122.1
• Coordinates: 2132506..2132763 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: W0Q7K7
• Locus tag: X781_RS10370
• Protein ID: WP_025236801.1
• Coordinates: 2132760..2133026 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
X781_RS10350	2127792..2128226	-	435	WP_025236797.1	D-tyrosyl-tRNA(Tyr) deacylase	-
X781_RS10355	2128201..2129043	-	843	WP_025236798.1	virulence factor BrkB family protein	-
X781_RS10360	2129045..2130283	-	1239	WP_025236799.1	glutamate-5-semialdehyde dehydrogenase	-
X781_RS10365	2130595..2132481	+	1887	WP_025236800.1	DNA mismatch repair endonuclease MutL	-
X781_RS12105	2132506..2132763	-	258	WP_081732122.1	type II toxin-antitoxin system YafQ family toxin	Toxin
X781_RS10370	2132760..2133026	-	267	WP_025236801.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
X781_RS10375	2133189..2134142	+	954	WP_025236802.1	tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA	-
X781_RS10380	2134266..2134541	+	276	WP_025236803.1	RNA chaperone Hfq	-
X781_RS10385	2134561..2135946	+	1386	WP_025236804.1	GTPase HflX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-70)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9869.37 Da
• Isoelectric Point: 7.2116

>T45794 WP_081732122.1 NZ_CP006942:c2132763-2132506 [Mannheimia sp. USDA-ARS-USMARC-1261]
MSYSNAFKRDYKKCAVDTLSSSLIEALYFLINDKPLPEKYCDHALTGEWKGFRDCHIQPDLVLIYRKIDDKLELLRIGSH
SNLFG

Nucleotide

• Length: 258 bp

>T45794 NZ_CP006942:c2132763-2132506 [Mannheimia sp. USDA-ARS-USMARC-1261]
TTGAGTTATAGTAACGCTTTTAAACGAGACTATAAAAAGTGTGCCGTTGATACATTATCTTCTTCGCTGATTGAAGCACT
CTATTTTCTCATCAACGATAAGCCATTACCTGAGAAATATTGCGATCACGCATTAACAGGTGAATGGAAAGGTTTTCGGG
ATTGCCATATTCAGCCTGATTTAGTGCTGATTTATCGAAAGATTGATGATAAATTAGAATTGTTGAGAATAGGTAGTCAT
TCCAATTTATTTGGGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9670.11 Da
• Isoelectric Point: 4.7863

>AT45794 WP_025236801.1 NZ_CP006942:c2133026-2132760 [Mannheimia sp. USDA-ARS-USMARC-1261]
MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATEKRFQLDYQPNVETAEVLKAVRNGDEKLNPAKNIADL
MEQLNARD

Nucleotide

• Length: 267 bp

>AT45794 NZ_CP006942:c2133026-2132760 [Mannheimia sp. USDA-ARS-USMARC-1261]
ATGGCAAATAGTGCATTAGTTAGTGTAAGAATTAGCCCTGAAATTAAAGAGCAGGCAAGCGAAGTTTTAGCCGGAATTGG
TTTAACGGTTTCTGATGTTATGCGAATGACATTAGCAAAAATTGCGACGGAAAAACGTTTTCAGCTTGACTATCAACCTA
ACGTGGAAACAGCAGAAGTATTAAAAGCGGTTAGAAATGGAGATGAAAAATTAAATCCGGCTAAAAACATTGCTGATTTA
ATGGAGCAACTTAATGCGAGAGATTGA

Structures

Antitoxin

• AlphaFold DB: W0Q7K7