Detailed information of TA system TA045792

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1066257..1066874
• Replicon: chromosome
• Accession: NZ_CP006942
• Organism: Mannheimia sp. USDA-ARS-USMARC-1261

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: W0Q2S3
• Locus tag: X781_RS05210
• Protein ID: WP_025235838.1
• Coordinates: 1066257..1066439 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: X781_RS05215
• Protein ID: WP_025235839.1
• Coordinates: 1066464..1066874 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
X781_RS05200	1062686..1064452	+	1767	WP_025235836.1	cysteine/glutathione ABC transporter permease/ATP-binding protein CydD	-
X781_RS05205	1064452..1066119	+	1668	WP_025235837.1	cysteine/glutathione ABC transporter ATP-binding protein/permease CydC	-
X781_RS05210	1066257..1066439	+	183	WP_025235838.1	type II toxin-antitoxin system HicA family toxin	Toxin
X781_RS05215	1066464..1066874	+	411	WP_025235839.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
X781_RS05220	1066991..1067620	+	630	WP_025235840.1	high frequency lysogenization protein HflD	-
X781_RS05225	1067654..1069021	+	1368	WP_025235841.1	adenylosuccinate lyase	-
X781_RS05230	1069088..1069849	+	762	WP_025235842.1	DeoR/GlpR family transcriptional regulator	-
X781_RS05235	1069842..1070717	+	876	WP_025235843.1	4-hydroxybenzoate octaprenyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6681.85 Da
• Isoelectric Point: 10.9132

>T45792 WP_025235838.1 NZ_CP006942:1066257-1066439 [Mannheimia sp. USDA-ARS-USMARC-1261]
MDSRSLVKLIVADGWFLVGIKGSHHQYKHSTKKGRVTFPHPKKDLPTATVKSILKQAGLE

Nucleotide

• Length: 183 bp

>T45792 NZ_CP006942:1066257-1066439 [Mannheimia sp. USDA-ARS-USMARC-1261]
ATGGATAGTCGAAGTTTAGTAAAATTAATCGTTGCAGACGGTTGGTTTTTAGTTGGAATTAAAGGTAGTCATCATCAATA
TAAACACTCAACTAAAAAAGGTCGTGTAACGTTTCCTCATCCGAAAAAAGATTTACCTACTGCGACAGTTAAGTCAATAT
TAAAACAAGCTGGTCTGGAATAG

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15251.50 Da
• Isoelectric Point: 4.6438

>AT45792 WP_025235839.1 NZ_CP006942:1066464-1066874 [Mannheimia sp. USDA-ARS-USMARC-1261]
MLYPIAIEIGNEKPSYGVIVPDVKGCFSAGDTLEEAFNNAKEAILFHIETLIEDGEVIPQPTALENHKNNPEYKDFIFAL
VDVDLTHLMGKTEKINVTLPSLLIRRIDEFVAKNPVYKTRSGFLAQVASERILTNY

Nucleotide

• Length: 411 bp

>AT45792 NZ_CP006942:1066464-1066874 [Mannheimia sp. USDA-ARS-USMARC-1261]
ATGTTATATCCTATTGCAATTGAAATAGGGAATGAAAAACCATCCTATGGAGTCATTGTTCCTGATGTGAAAGGCTGTTT
TTCAGCAGGAGATACATTAGAAGAGGCTTTTAATAATGCTAAAGAGGCTATTTTATTCCATATTGAAACATTAATTGAAG
ATGGTGAAGTTATCCCACAACCGACAGCACTTGAAAATCATAAAAATAATCCTGAATACAAAGATTTTATTTTCGCTTTA
GTAGATGTAGATTTAACTCATTTAATGGGAAAAACTGAAAAAATCAATGTGACTTTACCGTCATTACTCATTCGCCGTAT
TGATGAGTTTGTTGCTAAAAATCCAGTGTATAAAACCCGAAGCGGTTTTTTAGCTCAAGTGGCAAGTGAACGTATTTTAA
CAAATTACTAG

Structures

Toxin

• AlphaFold DB: W0Q2S3