Detailed information of TA system TA045789

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-YefM
• Location: 254061..254749
• Replicon: chromosome
• Accession: NZ_CP006942
• Organism: Mannheimia sp. USDA-ARS-USMARC-1261

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: W0Q2A7
• Locus tag: X781_RS01245
• Protein ID: WP_025235180.1
• Coordinates: 254327..254749 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: W0Q1I7
• Locus tag: X781_RS01240
• Protein ID: WP_025235179.1
• Coordinates: 254061..254327 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
X781_RS01220	249835..250479	-	645	WP_025235176.1	RNA pyrophosphohydrolase	-
X781_RS01225	251088..252233	+	1146	Protein_231	FMN-dependent L-lactate dehydrogenase LldD	-
X781_RS01230	252274..252933	-	660	WP_025235177.1	NAD(P)H-binding protein	-
X781_RS01235	252981..253829	-	849	WP_025235178.1	MBL fold metallo-hydrolase	-
X781_RS01240	254061..254327	+	267	WP_025235179.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
X781_RS01245	254327..254749	+	423	WP_025235180.1	type II toxin-antitoxin system VapC family toxin	Toxin
X781_RS01250	254749..255639	+	891	WP_025235181.1	LysR family transcriptional regulator	-
X781_RS01255	255768..256826	-	1059	WP_025235182.1	NAD(P)-dependent alcohol dehydrogenase	-
X781_RS01260	256960..257619	-	660	WP_025235183.1	NAD(P)H-dependent oxidoreductase	-
X781_RS01265	257644..258454	-	811	Protein_239	NADPH dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-58)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15907.45 Da
• Isoelectric Point: 7.5204

>T45789 WP_025235180.1 NZ_CP006942:254327-254749 [Mannheimia sp. USDA-ARS-USMARC-1261]
MFLLDTNVVSELRKVKKGMANPNVVAWLAKQNPNDFYINSIVLMELERGVLAMERKDPTQGVHLRHWQNLFLNQILHKPI
LPIDETTAQICAKLHIPDHAPENDAWIAASAIQHHLILVTRNTADFAKTGAKLFNPFEPQ

Nucleotide

• Length: 423 bp

>T45789 NZ_CP006942:254327-254749 [Mannheimia sp. USDA-ARS-USMARC-1261]
ATGTTTTTACTCGATACTAATGTGGTCAGCGAACTGCGAAAAGTGAAAAAAGGGATGGCTAATCCAAATGTAGTAGCGTG
GTTGGCAAAGCAAAATCCGAATGATTTTTACATTAATTCTATTGTGCTAATGGAACTGGAACGGGGCGTTTTGGCGATGG
AGCGAAAAGATCCTACGCAAGGTGTTCATCTACGCCATTGGCAAAATCTGTTTCTCAACCAAATTTTGCATAAGCCGATT
TTACCCATTGACGAAACCACCGCCCAAATCTGTGCCAAACTGCATATTCCCGATCACGCCCCTGAAAATGATGCGTGGAT
TGCAGCCTCCGCTATTCAACACCATTTGATTTTGGTTACTCGCAATACAGCTGATTTCGCCAAAACAGGGGCAAAATTGT
TTAACCCCTTTGAGCCACAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10025.41 Da
• Isoelectric Point: 6.7166

>AT45789 WP_025235179.1 NZ_CP006942:254061-254327 [Mannheimia sp. USDA-ARS-USMARC-1261]
MSVMTCREFNQKTSLAQKYAETAPVIITHRGKPTFVLLKYEDYQPQKTARSALHALLDLDHSPDVADIELELKPRSRAQR
PPVDFGDE

Nucleotide

• Length: 267 bp

>AT45789 NZ_CP006942:254061-254327 [Mannheimia sp. USDA-ARS-USMARC-1261]
ATGAGTGTAATGACCTGCCGTGAATTTAATCAAAAAACCAGCCTTGCCCAAAAATATGCGGAGACCGCACCGGTGATTAT
TACCCATCGAGGTAAGCCGACTTTTGTGTTGTTGAAGTATGAGGATTATCAACCGCAAAAAACCGCTCGAAGTGCCTTAC
ACGCTTTGTTAGATCTGGATCATTCCCCCGATGTGGCGGATATTGAGTTGGAGTTAAAGCCCCGTAGTCGAGCCCAACGT
CCGCCGGTCGATTTTGGAGATGAGTGA

Structures

Toxin

• AlphaFold DB: W0Q2A7

Antitoxin

• AlphaFold DB: W0Q1I7