Detailed information of TA system TA045773

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 576569..577095
• Replicon: chromosome
• Accession: NZ_CP006929
• Organism: Dickeya zeae EC1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: W909_RS02640
• Protein ID: WP_016943176.1
• Coordinates: 576569..576874 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: E0SK21
• Locus tag: W909_RS02645
• Protein ID: WP_012773205.1
• Coordinates: 576877..577095 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
W909_RS02610	571991..572422	+	432	WP_016943182.1	Cd(II)/Pb(II)-responsive transcriptional regulator	-
W909_RS02615	572497..573519	-	1023	WP_016943181.1	LacI family DNA-binding transcriptional regulator	-
W909_RS02620	573801..575000	+	1200	WP_029456553.1	L-talarate/galactarate dehydratase	-
W909_RS19910	575101..575316	-	216	Protein_514	transposase	-
W909_RS02625	575351..575626	-	276	Protein_515	transposase	-
W909_RS02640	576569..576874	-	306	WP_016943176.1	type II toxin-antitoxin system toxin CcdB	Toxin
W909_RS02645	576877..577095	-	219	WP_012773205.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
W909_RS02660	577768..578343	-	576	WP_016943174.1	transcriptional regulator	-
W909_RS02665	578436..580199	-	1764	WP_016943173.1	protein-disulfide reductase DsbD	-
W909_RS02670	580175..580549	-	375	WP_029456550.1	divalent cation tolerance protein CutA	-
W909_RS02675	580706..582007	-	1302	WP_016943171.1	anaerobic C4-dicarboxylate transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	575351..575584	233

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11611.40 Da
• Isoelectric Point: 4.4829

>T45773 WP_016943176.1 NZ_CP006929:c576874-576569 [Dickeya zeae EC1]
MQFIVYQYKRASHYKMFIDVQSDIVDTPGRRMVIPLIEAHHLSDKVNKTLFPLISIDGESYRLMTTELSSVPVEVMGEEI
ADLGDYADEIKDAINLMFWGI

Nucleotide

• Length: 306 bp

>T45773 NZ_CP006929:c576874-576569 [Dickeya zeae EC1]
ATGCAATTCATTGTTTATCAATACAAGCGCGCCAGCCACTACAAAATGTTTATCGATGTACAAAGTGATATTGTCGATAC
GCCAGGGCGACGAATGGTCATTCCGCTTATCGAAGCACATCACCTGTCTGATAAAGTGAATAAAACATTGTTCCCTCTAA
TCAGCATCGACGGCGAGAGCTATCGACTGATGACCACTGAGCTATCGAGCGTTCCCGTTGAAGTCATGGGTGAAGAGATC
GCGGATCTTGGCGACTATGCGGATGAGATCAAGGATGCTATCAATCTTATGTTTTGGGGGATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8201.16 Da
• Isoelectric Point: 7.2038

>AT45773 WP_012773205.1 NZ_CP006929:c577095-576877 [Dickeya zeae EC1]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEEWKKENREGMEEVARFIAQHGSFADENRNW

Nucleotide

• Length: 219 bp

>AT45773 NZ_CP006929:c577095-576877 [Dickeya zeae EC1]
ATGAAACACCGCGTTAGCGTTACCGTGGACAAAGACAATTATCAGGTTCTGAGTGCTGCCGGAGTCAATATTTCCGGGCT
GGTGAATGACGCCATTGGCAAGGAAGCCCGCCGCATCAAGGCTGAGGAGTGGAAGAAGGAAAACCGCGAAGGGATGGAAG
AAGTCGCCCGATTTATCGCACAGCATGGCTCCTTTGCGGATGAAAACAGGAACTGGTGA

Structures

Antitoxin

• AlphaFold DB: E0SK21