Detailed information of TA system TA045770

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 456309..456836
• Replicon: chromosome
• Accession: NZ_CP006929
• Organism: Dickeya zeae EC1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: W909_RS02070
• Protein ID: WP_016943951.1
• Coordinates: 456561..456836 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D2C2R6
• Locus tag: W909_RS02065
• Protein ID: WP_012883160.1
• Coordinates: 456309..456557 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
W909_RS02035	451625..452452	+	828	WP_033581532.1	ribbon-helix-helix domain-containing protein	-
W909_RS02040	452592..453458	+	867	WP_016943946.1	GTPase family protein	-
W909_RS02045	453545..454366	+	822	WP_016943947.1	DUF945 domain-containing protein	-
W909_RS02050	454396..454869	+	474	WP_016943948.1	DNA repair protein RadC	-
W909_RS02055	454933..455247	+	315	WP_016943949.1	TA system toxin CbtA family protein	-
W909_RS02060	455365..456198	+	834	WP_016943950.1	DUF4942 domain-containing protein	-
W909_RS02065	456309..456557	+	249	WP_012883160.1	ribbon-helix-helix domain-containing protein	Antitoxin
W909_RS02070	456561..456836	+	276	WP_016943951.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
W909_RS20475	456920..457603	-	684	Protein_403	IS1 family transposase	-
W909_RS02080	457738..460122	+	2385	WP_016943953.1	dynamin family protein	-
W909_RS02085	460119..461024	+	906	WP_016943954.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	445905..471426	25521
	-	flank	IS/Tn	-	-	457403..457603	200

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-43)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 11164.49 Da
• Isoelectric Point: 5.5403

>T45770 WP_016943951.1 NZ_CP006929:456561-456836 [Dickeya zeae EC1]
MEILWTQKAQDDLERIYRFASQYSRQHADDVLDRLFIGTTELVDHPRIGVSQTRYEPREVRKILFGDYEVHYEIQHNTIY
IIDLWHTREER

Nucleotide

• Length: 276 bp

>T45770 NZ_CP006929:456561-456836 [Dickeya zeae EC1]
ATGGAAATACTTTGGACACAAAAGGCACAGGATGACCTAGAGCGCATTTATCGGTTTGCCAGCCAGTACAGTCGGCAGCA
TGCTGACGATGTGTTAGATCGCCTGTTCATCGGAACAACAGAACTTGTTGATCATCCAAGAATCGGTGTATCTCAGACAA
GATATGAACCCCGTGAGGTGAGAAAAATTCTGTTTGGTGATTACGAGGTTCACTATGAAATTCAGCACAACACTATTTAT
ATCATCGATCTTTGGCATACACGAGAGGAGCGTTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9020.34 Da
• Isoelectric Point: 6.4653

>AT45770 WP_012883160.1 NZ_CP006929:456309-456557 [Dickeya zeae EC1]
MGQPAKQTISAQIPAELAAAVESLAIELDRSKSWVIKEALTAMIEERERQHQRILQGLADVDAGRVVNHADVLDFANKLK
KS

Nucleotide

• Length: 249 bp

>AT45770 NZ_CP006929:456309-456557 [Dickeya zeae EC1]
ATGGGACAGCCCGCAAAACAGACTATTAGTGCTCAGATACCCGCCGAGCTTGCTGCTGCGGTTGAAAGCCTGGCTATTGA
ATTGGATAGGTCGAAAAGTTGGGTGATTAAAGAAGCATTGACCGCAATGATCGAAGAGAGAGAACGTCAGCATCAACGGA
TTTTGCAAGGCCTTGCTGATGTTGATGCTGGAAGGGTGGTAAACCATGCTGATGTTCTCGATTTTGCCAATAAACTGAAA
AAGTCGTAA

Structures

Antitoxin

• AlphaFold DB: A0A375A6R5