Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 36321..37057 Replicon plasmid pNJST258N1
Accession NZ_CP006927
Organism Klebsiella pneumoniae 30660/NJST258_1

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag KPNJ1_RS27330 Protein ID WP_003026803.1
Coordinates 36321..36803 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KPNJ1_RS27335 Protein ID WP_003026799.1
Coordinates 36791..37057 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNJ1_RS27310 31958..32920 + 963 WP_004152113.1 M48 family metalloprotease -
KPNJ1_RS30315 33008..34262 + 1255 Protein_29 IS3 family transposase -
KPNJ1_RS27325 34763..36109 - 1347 WP_020314316.1 ISNCY family transposase -
KPNJ1_RS27330 36321..36803 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
KPNJ1_RS27335 36791..37057 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KPNJ1_RS27340 37233..37487 - 255 WP_004152108.1 hypothetical protein -
KPNJ1_RS27345 37563..37820 - 258 WP_004152107.1 hypothetical protein -
KPNJ1_RS27350 37869..38072 - 204 WP_004152106.1 hemolysin expression modulator Hha -
KPNJ1_RS27355 38106..38474 - 369 WP_004152105.1 hypothetical protein -
KPNJ1_RS27360 38518..39012 - 495 WP_004152104.1 DNA-binding protein -
KPNJ1_RS27365 39043..39618 - 576 WP_004152103.1 hypothetical protein -
KPNJ1_RS27370 39606..39875 - 270 WP_004152102.1 hypothetical protein -
KPNJ1_RS27375 40233..40583 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
KPNJ1_RS27380 40633..40995 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..142788 142788


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T45768 WP_003026803.1 NZ_CP006927:c36803-36321 [Klebsiella pneumoniae 30660/NJST258_1]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T45768 NZ_CP006927:c36803-36321 [Klebsiella pneumoniae 30660/NJST258_1]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT45768 WP_003026799.1 NZ_CP006927:c37057-36791 [Klebsiella pneumoniae 30660/NJST258_1]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT45768 NZ_CP006927:c37057-36791 [Klebsiella pneumoniae 30660/NJST258_1]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References