Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 67826..68469 Replicon plasmid pNJST258N2
Accession NZ_CP006926
Organism Klebsiella pneumoniae 30660/NJST258_1

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag KPNJ1_RS27120 Protein ID WP_001044770.1
Coordinates 67826..68242 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag KPNJ1_RS27125 Protein ID WP_001261282.1
Coordinates 68239..68469 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNJ1_RS31735 63717..63782 + 66 Protein_68 EAL domain-containing protein -
KPNJ1_RS27090 63922..64686 - 765 WP_001389365.1 IS6-like element IS6100 family transposase -
KPNJ1_RS27095 64829..65095 - 267 WP_001144737.1 plasmid mobilization relaxosome protein MobC -
KPNJ1_RS27100 65316..65789 - 474 WP_004201280.1 trimethoprim-resistant dihydrofolate reductase DfrA14 -
KPNJ1_RS27105 65945..66712 + 768 Protein_72 class 1 integron integrase IntI1 -
KPNJ1_RS27110 66765..67469 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
KPNJ1_RS27115 67516..67752 - 237 WP_001348075.1 AAA family ATPase -
KPNJ1_RS27120 67826..68242 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
KPNJ1_RS27125 68239..68469 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
KPNJ1_RS31740 68426..68886 + 461 Protein_77 hypothetical protein -
KPNJ1_RS30540 69014..69334 + 321 WP_032072060.1 hypothetical protein -
KPNJ1_RS29715 69543..69731 + 189 WP_000957857.1 hypothetical protein -
KPNJ1_RS27135 69741..70940 + 1200 WP_000948429.1 IS91 family transposase -
KPNJ1_RS27140 71071..71709 + 639 WP_025404033.1 hypothetical protein -
KPNJ1_RS27145 71712..72497 + 786 WP_032072937.1 site-specific integrase -
KPNJ1_RS31745 72555..72812 - 258 WP_032408936.1 hypothetical protein -
KPNJ1_RS31750 72941..73054 - 114 WP_032072094.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-3 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 - 1..73636 73636
- inside IScluster/Tn blaKPC-3 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 - 36181..70940 34759


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T45767 WP_001044770.1 NZ_CP006926:c68242-67826 [Klebsiella pneumoniae 30660/NJST258_1]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T45767 NZ_CP006926:c68242-67826 [Klebsiella pneumoniae 30660/NJST258_1]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT45767 WP_001261282.1 NZ_CP006926:c68469-68239 [Klebsiella pneumoniae 30660/NJST258_1]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT45767 NZ_CP006926:c68469-68239 [Klebsiella pneumoniae 30660/NJST258_1]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References