Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5819..6344 Replicon plasmid pNJST258N3
Accession NZ_CP006925
Organism Klebsiella pneumoniae 30660/NJST258_1

Toxin (Protein)


Gene name ccdB Uniprot ID W8UQV6
Locus tag KPNJ1_RS26620 Protein ID WP_001568026.1
Coordinates 5819..6124 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag KPNJ1_RS26625 Protein ID WP_001568025.1
Coordinates 6126..6344 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNJ1_RS26595 1519..2145 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
KPNJ1_RS29675 2265..2444 + 180 WP_006788217.1 hypothetical protein -
KPNJ1_RS26600 2900..3694 - 795 WP_004197635.1 site-specific integrase -
KPNJ1_RS26605 3892..4908 - 1017 WP_020315256.1 hypothetical protein -
KPNJ1_RS26610 4905..5228 - 324 WP_022644730.1 hypothetical protein -
KPNJ1_RS26615 5255..5650 - 396 WP_017899885.1 hypothetical protein -
KPNJ1_RS26620 5819..6124 - 306 WP_001568026.1 type II toxin-antitoxin system toxin CcdB Toxin
KPNJ1_RS26625 6126..6344 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KPNJ1_RS26630 6897..7586 + 690 WP_004193995.1 hypothetical protein -
KPNJ1_RS26635 7618..8307 - 690 WP_020315560.1 RES family NAD+ phosphorylase -
KPNJ1_RS26640 8744..9013 + 270 WP_020804424.1 hypothetical protein -
KPNJ1_RS26645 9001..9576 + 576 WP_020317495.1 hypothetical protein -
KPNJ1_RS26650 9607..10101 + 495 WP_020802777.1 hypothetical protein -
KPNJ1_RS26655 10145..10513 + 369 WP_004197807.1 hypothetical protein -
KPNJ1_RS26660 10547..10750 + 204 WP_004197808.1 hemolysin expression modulator Hha -
KPNJ1_RS28025 10764..10967 + 204 WP_004197809.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..36109 36109


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11645.32 Da        Isoelectric Point: 5.6919

>T45766 WP_001568026.1 NZ_CP006925:c6124-5819 [Klebsiella pneumoniae 30660/NJST258_1]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T45766 NZ_CP006925:c6124-5819 [Klebsiella pneumoniae 30660/NJST258_1]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCTGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT45766 WP_001568025.1 NZ_CP006925:c6344-6126 [Klebsiella pneumoniae 30660/NJST258_1]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT45766 NZ_CP006925:c6344-6126 [Klebsiella pneumoniae 30660/NJST258_1]
ATGAAGCAGCGCATCACTGTCACCATAGACAGTGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EZN1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References