Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4049914..4050533 Replicon chromosome
Accession NZ_CP006923
Organism Klebsiella pneumoniae 30660/NJST258_1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KPNJ1_RS20485 Protein ID WP_002892050.1
Coordinates 4050315..4050533 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KPNJ1_RS20480 Protein ID WP_002892066.1
Coordinates 4049914..4050288 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNJ1_RS20470 4045066..4046259 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KPNJ1_RS20475 4046282..4049428 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KPNJ1_RS20480 4049914..4050288 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KPNJ1_RS20485 4050315..4050533 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
KPNJ1_RS20490 4050692..4051258 + 567 WP_002892030.1 maltose O-acetyltransferase -
KPNJ1_RS31475 4051230..4051370 - 141 WP_004147370.1 hypothetical protein -
KPNJ1_RS20500 4051391..4051861 + 471 WP_002892026.1 YlaC family protein -
KPNJ1_RS20505 4051836..4053287 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KPNJ1_RS20510 4053388..4054086 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
KPNJ1_RS20515 4054083..4054223 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KPNJ1_RS20520 4054223..4054486 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T45762 WP_002892050.1 NZ_CP006923:4050315-4050533 [Klebsiella pneumoniae 30660/NJST258_1]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T45762 NZ_CP006923:4050315-4050533 [Klebsiella pneumoniae 30660/NJST258_1]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT45762 WP_002892066.1 NZ_CP006923:4049914-4050288 [Klebsiella pneumoniae 30660/NJST258_1]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT45762 NZ_CP006923:4049914-4050288 [Klebsiella pneumoniae 30660/NJST258_1]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References