Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4956..5481 Replicon plasmid pNJST258C3
Accession NZ_CP006921
Organism Klebsiella pneumoniae 30684/NJST258_2

Toxin (Protein)


Gene name ccdB Uniprot ID W8UQV6
Locus tag KPNJ2_RS26845 Protein ID WP_001568026.1
Coordinates 5176..5481 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag KPNJ2_RS26840 Protein ID WP_001568025.1
Coordinates 4956..5174 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNJ2_RS27420 294..473 - 180 WP_050893391.1 hypothetical protein -
KPNJ2_RS26805 550..753 - 204 WP_004197808.1 hemolysin expression modulator Hha -
KPNJ2_RS26810 787..1155 - 369 WP_004197807.1 hypothetical protein -
KPNJ2_RS26815 1199..1693 - 495 WP_020802777.1 hypothetical protein -
KPNJ2_RS26820 1724..2299 - 576 WP_020317495.1 hypothetical protein -
KPNJ2_RS26825 2287..2556 - 270 WP_020804424.1 hypothetical protein -
KPNJ2_RS26830 2993..3682 + 690 WP_020315560.1 RES family NAD+ phosphorylase -
KPNJ2_RS26835 3714..4403 - 690 WP_004193995.1 hypothetical protein -
KPNJ2_RS26840 4956..5174 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KPNJ2_RS26845 5176..5481 + 306 WP_001568026.1 type II toxin-antitoxin system toxin CcdB Toxin
KPNJ2_RS26850 5650..6045 + 396 WP_017899885.1 hypothetical protein -
KPNJ2_RS26855 6072..6395 + 324 WP_022644730.1 hypothetical protein -
KPNJ2_RS26860 6392..7408 + 1017 WP_020315256.1 hypothetical protein -
KPNJ2_RS26865 7606..8400 + 795 WP_004197635.1 site-specific integrase -
KPNJ2_RS28880 8848..9027 - 180 WP_006788217.1 hypothetical protein -
KPNJ2_RS26875 9147..9773 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..12399 12399


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11645.32 Da        Isoelectric Point: 5.6919

>T45748 WP_001568026.1 NZ_CP006921:5176-5481 [Klebsiella pneumoniae 30684/NJST258_2]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T45748 NZ_CP006921:5176-5481 [Klebsiella pneumoniae 30684/NJST258_2]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCTGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT45748 WP_001568025.1 NZ_CP006921:4956-5174 [Klebsiella pneumoniae 30684/NJST258_2]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT45748 NZ_CP006921:4956-5174 [Klebsiella pneumoniae 30684/NJST258_2]
ATGAAGCAGCGCATCACTGTCACCATAGACAGTGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EZN1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References