Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4063187..4063806 Replicon chromosome
Accession NZ_CP006918
Organism Klebsiella pneumoniae 30684/NJST258_2

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KPNJ2_RS20500 Protein ID WP_002892050.1
Coordinates 4063588..4063806 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KPNJ2_RS20495 Protein ID WP_002892066.1
Coordinates 4063187..4063561 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPNJ2_RS20485 4058339..4059532 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KPNJ2_RS20490 4059555..4062701 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KPNJ2_RS20495 4063187..4063561 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KPNJ2_RS20500 4063588..4063806 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
KPNJ2_RS20505 4063965..4064531 + 567 WP_002892030.1 maltose O-acetyltransferase -
KPNJ2_RS30320 4064503..4064643 - 141 WP_004147370.1 hypothetical protein -
KPNJ2_RS20515 4064664..4065134 + 471 WP_002892026.1 YlaC family protein -
KPNJ2_RS20520 4065109..4066560 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KPNJ2_RS20525 4066661..4067359 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
KPNJ2_RS20530 4067356..4067496 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KPNJ2_RS20535 4067496..4067759 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T45743 WP_002892050.1 NZ_CP006918:4063588-4063806 [Klebsiella pneumoniae 30684/NJST258_2]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T45743 NZ_CP006918:4063588-4063806 [Klebsiella pneumoniae 30684/NJST258_2]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT45743 WP_002892066.1 NZ_CP006918:4063187-4063561 [Klebsiella pneumoniae 30684/NJST258_2]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT45743 NZ_CP006918:4063187-4063561 [Klebsiella pneumoniae 30684/NJST258_2]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References