Detailed information of TA system TA045729

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1256630..1257547
• Replicon: chromosome
• Accession: NZ_CP006890
• Organism: Bacillus velezensis SQR9

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: V529_RS06085
• Protein ID: WP_007407256.1
• Coordinates: 1256801..1257547 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: V529_RS06080
• Protein ID: WP_003154807.1
• Coordinates: 1256630..1256800 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
V529_RS06035	1251782..1252294	+	513	WP_032874577.1	sigma-70 family RNA polymerase sigma factor	-
V529_RS06040	1252407..1252628	+	222	Protein_1168	terminase	-
V529_RS20275	1253017..1253484	+	468	WP_076851257.1	hypothetical protein	-
V529_RS06050	1253497..1253868	+	372	WP_038457736.1	YomQ/XkdW protein, phage-like element PBSX	-
V529_RS06055	1253873..1254070	+	198	WP_007610833.1	XkdX family protein	-
V529_RS06060	1254127..1254888	+	762	WP_038457737.1	hypothetical protein	-
V529_RS06065	1254941..1255204	+	264	WP_003154815.1	hemolysin XhlA family protein	-
V529_RS06070	1255218..1255481	+	264	WP_003154813.1	phage holin	-
V529_RS06075	1255495..1256373	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
V529_RS06080	1256630..1256800	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
V529_RS06085	1256801..1257547	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
V529_RS06090	1257652..1258650	-	999	WP_032874611.1	inorganic phosphate transporter	-
V529_RS06095	1258663..1259280	-	618	WP_032874614.1	DUF47 domain-containing protein	-
V529_RS06100	1259565..1260881	-	1317	WP_038457739.1	amino acid permease	-
V529_RS06105	1261204..1262154	+	951	WP_038457742.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1231194..1314850	83656

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T45729 WP_007407256.1 NZ_CP006890:c1257547-1256801 [Bacillus velezensis SQR9]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T45729 NZ_CP006890:c1257547-1256801 [Bacillus velezensis SQR9]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCTGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT45729 WP_003154807.1 NZ_CP006890:c1256800-1256630 [Bacillus velezensis SQR9]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT45729 NZ_CP006890:c1256800-1256630 [Bacillus velezensis SQR9]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTTAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14