Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 470384..471021 Replicon chromosome
Accession NZ_CP006890
Organism Bacillus velezensis SQR9

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag V529_RS02330 Protein ID WP_003156187.1
Coordinates 470671..471021 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag V529_RS02325 Protein ID WP_003156188.1
Coordinates 470384..470665 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
V529_RS02305 466749..467348 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
V529_RS02310 467441..467806 + 366 WP_007609580.1 holo-ACP synthase -
V529_RS02315 467971..468978 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
V529_RS02320 469095..470264 + 1170 WP_038457028.1 alanine racemase -
V529_RS02325 470384..470665 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
V529_RS02330 470671..471021 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
V529_RS02335 471139..471960 + 822 WP_003156182.1 STAS domain-containing protein -
V529_RS02340 471965..472330 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
V529_RS02345 472333..472734 + 402 WP_003156178.1 anti-sigma regulatory factor -
V529_RS02350 472746..473753 + 1008 WP_007410233.1 PP2C family protein-serine/threonine phosphatase -
V529_RS02355 473817..474146 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
V529_RS02360 474143..474625 + 483 WP_003156173.1 anti-sigma B factor RsbW -
V529_RS02365 474591..475379 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
V529_RS02370 475379..475981 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T45728 WP_003156187.1 NZ_CP006890:470671-471021 [Bacillus velezensis SQR9]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T45728 NZ_CP006890:470671-471021 [Bacillus velezensis SQR9]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT45728 WP_003156188.1 NZ_CP006890:470384-470665 [Bacillus velezensis SQR9]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT45728 NZ_CP006890:470384-470665 [Bacillus velezensis SQR9]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References