Detailed information of TA system TA045724

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 434718..435318
• Replicon: plasmid pRgalR602b
• Accession: NZ_CP006879
• Organism: Rhizobium gallicum bv. gallicum R602

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A0B4XBR1
• Locus tag: RGR602_RS23005
• Protein ID: WP_040114367.1
• Coordinates: 435028..435318 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: RGR602_RS23000
• Protein ID: WP_040114366.1
• Coordinates: 434718..435026 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RGR602_RS22980	431168..432676	-	1509	WP_052451715.1	ATP-binding protein	-
RGR602_RS22990	433018..433509	+	492	WP_082046656.1	alanine racemase	-
RGR602_RS36295	433754..434522	+	769	Protein_422	IS30 family transposase	-
RGR602_RS23000	434718..435026	-	309	WP_040114366.1	putative addiction module antidote protein	Antitoxin
RGR602_RS23005	435028..435318	-	291	WP_040114367.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RGR602_RS23015	436502..437437	+	936	WP_040114369.1	hypothetical protein	-
RGR602_RS23020	437532..438182	+	651	WP_166677531.1	hypothetical protein	-
RGR602_RS37550	438744..438905	-	162	Protein_427	cytochrome C peroxidase	-
RGR602_RS37555	439412..439531	+	120	Protein_428	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..507254	507254

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-90)

Antitoxin

• Cro/C1-type helix-turn-helix domain (50-89)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11060.87 Da
• Isoelectric Point: 10.4419

>T45724 WP_040114367.1 NZ_CP006879:c435318-435028 [Rhizobium gallicum bv. gallicum R602]
MIEVRLTVTFSRWLEDLKDRRARDRVLARLRRFELENFGDVKPVGSGVSEARIDYGPGYRLYFVRQADVIVVMLCGGDKA
RQKRDIATAKAMAKEL

Nucleotide

• Length: 291 bp

>T45724 NZ_CP006879:c435318-435028 [Rhizobium gallicum bv. gallicum R602]
ATGATTGAAGTCCGCCTGACAGTCACTTTTTCCCGATGGCTGGAAGATCTGAAAGACCGGCGCGCGCGTGACAGAGTTCT
CGCCAGATTGCGTCGTTTCGAATTAGAAAACTTCGGAGATGTAAAGCCCGTGGGGAGCGGGGTAAGCGAGGCACGAATAG
ATTATGGGCCGGGTTATCGGCTGTATTTTGTGAGACAGGCTGATGTTATCGTGGTGATGCTGTGCGGTGGCGACAAAGCA
AGACAGAAACGTGACATTGCGACGGCGAAGGCCATGGCGAAGGAGCTATAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 10846.31 Da
• Isoelectric Point: 7.0095

>AT45724 WP_040114366.1 NZ_CP006879:c435026-434718 [Rhizobium gallicum bv. gallicum R602]
MALETTRFDIQNYIKTHEDQTGYLDAVLEDGDPALIAAAIGDIARARGASQFAKDSGLSRETIYKVFRPGGNPTLDTIAK
AVKVLGLRLSLAPASNSTKGPI

Nucleotide

• Length: 309 bp

>AT45724 NZ_CP006879:c435026-434718 [Rhizobium gallicum bv. gallicum R602]
ATGGCATTGGAAACAACGCGCTTCGATATTCAGAACTACATCAAAACCCACGAGGATCAGACTGGCTATCTCGACGCTGT
TCTAGAGGATGGAGACCCTGCTCTTATTGCAGCGGCCATCGGCGATATTGCCCGCGCACGCGGCGCATCACAATTCGCGA
AAGACAGCGGGCTCAGTCGTGAAACCATCTACAAGGTTTTTCGCCCAGGGGGAAATCCGACGCTCGATACGATCGCCAAA
GCGGTGAAAGTGCTTGGCCTGCGGCTGTCGCTGGCACCGGCATCTAACTCCACCAAAGGCCCGATCTGA

Structures

Toxin

• AlphaFold DB: A0A0B4XBR1