Detailed information of TA system TA045718

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 1503078..1503686
• Replicon: chromosome
• Accession: NZ_CP006872
• Organism: Paenibacillus polymyxa SQR-21

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: H6CFY1
• Locus tag: PPSQR21_RS06275
• Protein ID: WP_007429335.1
• Coordinates: 1503336..1503686 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: PPSQR21_RS06270
• Protein ID: WP_170970806.1
• Coordinates: 1503078..1503329 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPSQR21_RS06255	1499270..1499902	+	633	WP_025364303.1	LysE family transporter	-
PPSQR21_RS06260	1500013..1501290	+	1278	WP_025364304.1	outer membrane lipoprotein carrier protein LolA	-
PPSQR21_RS06265	1501558..1502754	+	1197	WP_025364305.1	alanine racemase	-
PPSQR21_RS06270	1503078..1503329	+	252	WP_170970806.1	ribbon-helix-helix protein, CopG family	Antitoxin
PPSQR21_RS06275	1503336..1503686	+	351	WP_007429335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PPSQR21_RS06280	1503865..1506087	+	2223	WP_025364306.1	RNA-binding transcriptional accessory protein	-
PPSQR21_RS25855	1506191..1506325	-	135	WP_016821163.1	cortex morphogenetic protein CmpA	-
PPSQR21_RS06285	1506468..1506980	+	513	WP_016821162.1	SprT family protein	-
PPSQR21_RS06300	1507359..1508234	-	876	WP_016818364.1	Cof-type HAD-IIB family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12822.77 Da
• Isoelectric Point: 5.6195

>T45718 WP_007429335.1 NZ_CP006872:1503336-1503686 [Paenibacillus polymyxa SQR-21]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T45718 NZ_CP006872:1503336-1503686 [Paenibacillus polymyxa SQR-21]
TTGATCGTAAAGCGCGGCGACGTTTTTTTTGCAGACCTTTCACCCGTAGTCGGTTCCGAGCAAGGTGGCGTCAGACCTGT
GCTGATCATTCAAAATGATATCGGCAACCGGTTTAGCCCTACCGTGATCGTAGCAGCCATCACAGCACAGATTCAGAAGG
CAAAGCTGCCTACACACGTGGAAATCGACGCGGCGACGCATGGCTTTGATCGTGATTCTGTTGTTCTTCTGGAGCAAATC
CGGACGATCGACAAGCAAAGGCTTACCGACAAAATCACCCATTTGGATGAAGAAACCATGCGCAAAGTGAGCGATTCGCT
ACAGATCAGTCTCGGTTTGATTGATTTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9465.86 Da
• Isoelectric Point: 6.4671

>AT45718 WP_170970806.1 NZ_CP006872:1503078-1503329 [Paenibacillus polymyxa SQR-21]
MISLPDHLLQEVDGIVAMENSNRSEFIRQAMKLYVGERKKRYIREAMQRGYMEMAKINLTMASEAFHAEEDANSTLGRTV
SGV

Nucleotide

• Length: 252 bp

>AT45718 NZ_CP006872:1503078-1503329 [Paenibacillus polymyxa SQR-21]
ATGATCAGTTTGCCAGATCATCTCTTGCAGGAAGTGGACGGGATCGTAGCTATGGAGAATTCCAACCGCAGTGAATTTAT
CAGGCAGGCAATGAAGCTTTATGTAGGTGAACGTAAAAAACGTTACATTCGTGAAGCTATGCAGCGTGGATACATGGAAA
TGGCTAAAATTAACCTGACCATGGCATCTGAAGCCTTTCACGCGGAGGAAGATGCAAACAGCACCTTGGGCCGTACAGTT
AGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: H6CFY1