Detailed information of TA system TA045709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-RelB
• Location: 6994649..6995168
• Replicon: chromosome
• Accession: NZ_CP006850
• Organism: Nocardia nova SH22a

Toxin (Protein)

• Gene name: relE
• Uniprot ID: W5TQ28
• Locus tag: NONO_RS31980
• Protein ID: WP_025352582.1
• Coordinates: 6994899..6995168 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: W5TQN0
• Locus tag: NONO_RS31975
• Protein ID: WP_025352581.1
• Coordinates: 6994649..6994906 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NONO_RS31950	6990351..6991190	-	840	WP_025352576.1	mycofactocin-coupled SDR family oxidoreductase	-
NONO_RS31955	6991471..6991719	-	249	WP_081769825.1	hypothetical protein	-
NONO_RS31960	6992042..6992632	+	591	WP_148307037.1	hypothetical protein	-
NONO_RS31965	6992716..6993939	+	1224	WP_025352579.1	DUF2236 domain-containing protein	-
NONO_RS31970	6994040..6994594	-	555	WP_202807949.1	GNAT family N-acetyltransferase	-
NONO_RS31975	6994649..6994906	+	258	WP_025352581.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
NONO_RS31980	6994899..6995168	+	270	WP_025352582.1	Txe/YoeB family addiction module toxin	Toxin
NONO_RS31985	6995195..6996400	-	1206	WP_025352583.1	FAD-dependent monooxygenase	-
NONO_RS31990	6996411..6997289	-	879	WP_038555432.1	alpha/beta hydrolase	-
NONO_RS31995	6997438..6998121	-	684	WP_158436400.1	GAP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-66)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10120.57 Da
• Isoelectric Point: 9.4818

>T45709 WP_025352582.1 NZ_CP006850:6994899-6995168 [Nocardia nova SH22a]
MTERKLVFTAYGWETYTSWLTRDRTILKAANKLINAVLADPFTGIGKPEPLKHQLAGNWSRRISREHRLIYAVTDTEIIV
VGVGGNYDD

Nucleotide

• Length: 270 bp

>T45709 NZ_CP006850:6994899-6995168 [Nocardia nova SH22a]
ATGACTGAGCGCAAGCTCGTCTTCACCGCCTACGGGTGGGAGACCTACACATCGTGGCTCACACGTGATCGCACGATTCT
GAAGGCAGCCAACAAGCTGATCAACGCGGTGCTCGCCGATCCGTTCACCGGCATCGGTAAACCCGAGCCACTCAAACATC
AACTTGCCGGAAACTGGTCCCGCCGAATATCCCGAGAGCACCGCCTGATCTACGCCGTGACCGACACCGAGATCATCGTC
GTCGGCGTCGGCGGCAACTACGACGACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9372.36 Da
• Isoelectric Point: 4.0932

>AT45709 WP_025352581.1 NZ_CP006850:6994649-6994906 [Nocardia nova SH22a]
MTALPISTVRQQLFSLVQQVNDDHDTVTVVTKSGENAVLVSEADWNSIQETLYVLATHGGVRLLESARAAERGEFEAREL
LDTDD

Nucleotide

• Length: 258 bp

>AT45709 NZ_CP006850:6994649-6994906 [Nocardia nova SH22a]
ATGACCGCATTACCGATCTCGACCGTTCGCCAGCAGCTGTTCTCGCTGGTGCAGCAGGTCAACGATGACCACGACACGGT
CACCGTCGTGACCAAGAGCGGCGAGAACGCTGTGCTCGTATCCGAGGCGGACTGGAACTCCATTCAGGAAACGCTGTACG
TCCTGGCCACTCACGGTGGAGTTCGTCTGCTCGAATCCGCACGTGCGGCGGAACGAGGCGAGTTCGAGGCGCGCGAACTG
CTGGATACCGATGACTGA

Structures

Toxin

• AlphaFold DB: W5TQ28

Antitoxin

• AlphaFold DB: W5TQN0