Detailed information of TA system TA045708

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 2151506..2152173
• Replicon: chromosome
• Accession: NZ_CP006850
• Organism: Nocardia nova SH22a

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: W5TBM5
• Locus tag: NONO_RS09725
• Protein ID: WP_025348252.1
• Coordinates: 2151754..2152173 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: W5TC52
• Locus tag: NONO_RS09720
• Protein ID: WP_025348251.1
• Coordinates: 2151506..2151757 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NONO_RS09695	2147080..2147646	+	567	WP_025348246.1	NAD(P)H-dependent oxidoreductase	-
NONO_RS09700	2147807..2147929	+	123	WP_025348247.1	type B 50S ribosomal protein L36	-
NONO_RS09705	2148465..2148698	+	234	WP_025348248.1	redoxin NrdH	-
NONO_RS09710	2148741..2149244	+	504	WP_051494661.1	class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI	-
NONO_RS09715	2149196..2151385	+	2190	WP_193365200.1	class 1b ribonucleoside-diphosphate reductase subunit alpha	-
NONO_RS09720	2151506..2151757	+	252	WP_025348251.1	Arc family DNA-binding protein	Antitoxin
NONO_RS09725	2151754..2152173	+	420	WP_025348252.1	type II toxin-antitoxin system VapC family toxin	Toxin
NONO_RS09730	2152673..2154091	+	1419	WP_081769663.1	magnesium transporter	-
NONO_RS09735	2154371..2155333	+	963	WP_025348254.1	class 1b ribonucleoside-diphosphate reductase subunit beta	-
NONO_RS38805	2155931..2156302	+	372	WP_081769664.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-127)

Antitoxin

• Arc-like DNA binding domain (9-41)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15090.43 Da
• Isoelectric Point: 10.0786

>T45708 WP_025348252.1 NZ_CP006850:2151754-2152173 [Nocardia nova SH22a]
VIVLDTNVISEPLRKAPDRRVIDWIDSQELRNLYLTAITVAELRFGVASMSSGKRRDTLRDRLETRVLPLFTGRVLPFDL
DAAAAYAELMAAARSAGAAIGMADGYIAATAIRAKMAVATRDTSPFRAAGVSVIDPWAR

Nucleotide

• Length: 420 bp

>T45708 NZ_CP006850:2151754-2152173 [Nocardia nova SH22a]
GTGATCGTCCTGGACACCAATGTCATCAGCGAGCCGCTCCGCAAGGCGCCCGACCGCCGCGTGATCGACTGGATCGACAG
CCAGGAACTGCGCAATCTGTATCTGACCGCGATCACGGTGGCAGAGTTGCGCTTCGGTGTTGCGAGCATGTCCTCGGGAA
AGCGACGAGATACCCTGCGCGACAGGCTCGAAACCCGAGTTCTTCCGCTGTTCACCGGACGTGTTCTGCCATTCGACCTG
GACGCGGCGGCTGCTTACGCGGAACTCATGGCTGCTGCGAGATCCGCCGGTGCGGCGATAGGCATGGCCGACGGGTATAT
CGCTGCCACCGCGATCCGGGCGAAAATGGCGGTGGCCACCCGAGACACGTCGCCGTTCCGCGCTGCGGGCGTATCCGTCA
TCGATCCGTGGGCTCGATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9135.24 Da
• Isoelectric Point: 4.3535

>AT45708 WP_025348251.1 NZ_CP006850:2151506-2151757 [Nocardia nova SH22a]
MARLTVRDLPDEVREALRVRAAHNGRSTEAEVRAILIETVSPGSDVRLGSLLADIGREIELTDEEAEALAARDRTPPTPL
EFE

Nucleotide

• Length: 252 bp

>AT45708 NZ_CP006850:2151506-2151757 [Nocardia nova SH22a]
ATGGCCAGACTGACAGTCAGAGATCTACCCGACGAGGTCAGGGAAGCCTTGCGAGTGCGCGCCGCGCACAACGGTCGCAG
CACCGAGGCCGAGGTCAGGGCCATTCTCATCGAGACCGTTTCCCCCGGCTCGGACGTCAGGCTCGGCTCGTTACTCGCCG
ATATCGGTCGCGAGATCGAGTTGACCGATGAGGAAGCGGAAGCCCTCGCCGCCCGCGATCGCACACCCCCGACCCCGCTG
GAGTTCGAGTGA

Structures

Toxin

• AlphaFold DB: W5TBM5

Antitoxin

• AlphaFold DB: W5TC52