Detailed information of TA system TA045704

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4520094..4520929
• Replicon: chromosome
• Accession: NZ_CP006834
• Organism: Escherichia coli APEC O2-211

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1V3VXN0
• Locus tag: APECO2_RS23635
• Protein ID: WP_000854801.1
• Coordinates: 4520094..4520471 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1V3VXQ4
• Locus tag: APECO2_RS23640
• Protein ID: WP_001360188.1
• Coordinates: 4520561..4520929 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APECO2_RS23605	4515745..4517586	+	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-
APECO2_RS23610	4517664..4518170	-	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
APECO2_RS23620	4518470..4519315	-	846	Protein_4313	DUF4942 domain-containing protein	-
APECO2_RS23625	4519400..4519597	-	198	WP_000839269.1	DUF957 domain-containing protein	-
APECO2_RS23630	4519609..4520097	-	489	WP_000761658.1	hypothetical protein	-
APECO2_RS23635	4520094..4520471	-	378	WP_000854801.1	type IV toxin-antitoxin system toxin CbtA	Toxin
APECO2_RS23640	4520561..4520929	-	369	WP_001360188.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
APECO2_RS23645	4520979..4521623	-	645	WP_000086744.1	hypothetical protein	-
APECO2_RS23650	4521638..4521859	-	222	WP_000692334.1	DUF987 domain-containing protein	-
APECO2_RS23655	4521928..4522404	-	477	WP_001186725.1	RadC family protein	-
APECO2_RS23660	4522420..4522905	-	486	WP_000214418.1	antirestriction protein	-
APECO2_RS23665	4522996..4523814	-	819	WP_001234702.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13881.87 Da
• Isoelectric Point: 7.3219

>T45704 WP_000854801.1 NZ_CP006834:c4520471-4520094 [Escherichia coli APEC O2-211]
MKTLPVLPGQAASSRPSPVEIWQILLSQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T45704 NZ_CP006834:c4520471-4520094 [Escherichia coli APEC O2-211]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCAACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13563.27 Da
• Isoelectric Point: 6.2044

>AT45704 WP_001360188.1 NZ_CP006834:c4520929-4520561 [Escherichia coli APEC O2-211]
VSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGVTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT45704 NZ_CP006834:c4520929-4520561 [Escherichia coli APEC O2-211]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCATGACCGTCCCTGGTGGGGACTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGGTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1V3VXN0

Antitoxin

• AlphaFold DB: A0A1V3VXQ4