Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4191294..4191948 Replicon chromosome
Accession NZ_CP006834
Organism Escherichia coli APEC O2-211

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag APECO2_RS21875 Protein ID WP_000244781.1
Coordinates 4191294..4191701 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag APECO2_RS21880 Protein ID WP_000354046.1
Coordinates 4191682..4191948 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
APECO2_RS21855 4187251..4188984 - 1734 WP_000813191.1 single-stranded-DNA-specific exonuclease RecJ -
APECO2_RS21860 4188990..4189700 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
APECO2_RS21865 4189725..4190621 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
APECO2_RS21870 4190733..4191254 + 522 WP_001055874.1 flavodoxin FldB -
APECO2_RS21875 4191294..4191701 - 408 WP_000244781.1 protein YgfX Toxin
APECO2_RS21880 4191682..4191948 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
APECO2_RS21890 4192201..4193181 + 981 WP_000886050.1 tRNA-modifying protein YgfZ -
APECO2_RS21895 4193258..4193917 - 660 WP_000250269.1 hemolysin III family protein -
APECO2_RS21900 4194081..4194392 - 312 WP_001182953.1 N(4)-acetylcytidine aminohydrolase -
APECO2_RS21905 4194437..4195870 + 1434 WP_001389468.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T45701 WP_000244781.1 NZ_CP006834:c4191701-4191294 [Escherichia coli APEC O2-211]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T45701 NZ_CP006834:c4191701-4191294 [Escherichia coli APEC O2-211]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT45701 WP_000354046.1 NZ_CP006834:c4191948-4191682 [Escherichia coli APEC O2-211]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT45701 NZ_CP006834:c4191948-4191682 [Escherichia coli APEC O2-211]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References