Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3752292..3752917 Replicon chromosome
Accession NZ_CP006834
Organism Escherichia coli APEC O2-211

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag APECO2_RS19810 Protein ID WP_000911329.1
Coordinates 3752292..3752690 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag APECO2_RS19815 Protein ID WP_000450524.1
Coordinates 3752690..3752917 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
APECO2_RS19790 3748199..3748399 + 201 WP_000383836.1 YpfN family protein -
APECO2_RS19795 3748480..3749178 - 699 WP_000679819.1 esterase -
APECO2_RS19800 3749252..3751267 - 2016 WP_047340803.1 tRNA cytosine(34) acetyltransferase TmcA -
APECO2_RS19805 3751282..3752145 - 864 WP_001267498.1 neutral zinc metallopeptidase -
APECO2_RS19810 3752292..3752690 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
APECO2_RS19815 3752690..3752917 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
APECO2_RS19820 3753074..3753787 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
APECO2_RS19825 3754000..3755034 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
APECO2_RS19830 3755051..3755929 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
APECO2_RS19835 3756075..3756647 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
APECO2_RS19840 3756647..3757117 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T45700 WP_000911329.1 NZ_CP006834:c3752690-3752292 [Escherichia coli APEC O2-211]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T45700 NZ_CP006834:c3752690-3752292 [Escherichia coli APEC O2-211]
ATGCTGAAATTCATGCTGGATACCAACATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT45700 WP_000450524.1 NZ_CP006834:c3752917-3752690 [Escherichia coli APEC O2-211]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT45700 NZ_CP006834:c3752917-3752690 [Escherichia coli APEC O2-211]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References