Detailed information of TA system TA045689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2588426..2589062
• Replicon: chromosome
• Accession: NZ_CP006834
• Organism: Escherichia coli APEC O2-211

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A5F1EUB3
• Locus tag: APECO2_RS13705
• Protein ID: WP_000813796.1
• Coordinates: 2588426..2588602 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: APECO2_RS13710
• Protein ID: WP_076838470.1
• Coordinates: 2588646..2589062 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APECO2_RS13685	2584048..2585259	-	1212	WP_071998543.1	BenE family transporter YdcO	-
APECO2_RS13690	2585312..2585848	+	537	WP_000429147.1	DNA-binding transcriptional regulator SutR	-
APECO2_RS13695	2585921..2587882	+	1962	WP_001317809.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
APECO2_RS13700	2587974..2588204	-	231	WP_000494243.1	YncJ family protein	-
APECO2_RS13705	2588426..2588602	+	177	WP_000813796.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
APECO2_RS13710	2588646..2589062	+	417	WP_076838470.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
APECO2_RS13715	2589141..2590547	+	1407	WP_000760605.1	PLP-dependent aminotransferase family protein	-
APECO2_RS13720	2590792..2591937	+	1146	WP_000047448.1	ABC transporter substrate-binding protein	-
APECO2_RS13725	2591955..2592968	+	1014	WP_000220433.1	ABC transporter ATP-binding protein	-
APECO2_RS13730	2592969..2593910	+	942	WP_001251307.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6751.78 Da
• Isoelectric Point: 11.5666

>T45689 WP_000813796.1 NZ_CP006834:2588426-2588602 [Escherichia coli APEC O2-211]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLN

Nucleotide

• Length: 177 bp

>T45689 NZ_CP006834:2588426-2588602 [Escherichia coli APEC O2-211]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTAAATTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15219.59 Da
• Isoelectric Point: 4.5908

>AT45689 WP_076838470.1 NZ_CP006834:2588646-2589062 [Escherichia coli APEC O2-211]
MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT45689 NZ_CP006834:2588646-2589062 [Escherichia coli APEC O2-211]
ATGCGTTATCCCGTCACTCTTACTCCTGCGCCGGAAGGCGGTTATGTGGTTTCTTTTGTAGATATCCCTGAAGCGTTGAC
GCAGGGAGAAACTGTTGCTGAAGCGATGGAAGCAGCAAAAGATGCCTTACTCACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCTTATTTAACTTGCACCATGCTACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Structures

Toxin

• AlphaFold DB: A0A5F1EUB3