Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1516147..1516765 Replicon chromosome
Accession NZ_CP006834
Organism Escherichia coli APEC O2-211

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag APECO2_RS07860 Protein ID WP_001291435.1
Coordinates 1516147..1516365 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag APECO2_RS07865 Protein ID WP_000344800.1
Coordinates 1516391..1516765 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
APECO2_RS07825 1511436..1512008 + 573 WP_000779821.1 YbaY family lipoprotein -
APECO2_RS07830 1512039..1512350 - 312 WP_000409911.1 MGMT family protein -
APECO2_RS07840 1512729..1513082 + 354 WP_000878140.1 DUF1428 domain-containing protein -
APECO2_RS07845 1513124..1514674 - 1551 WP_001317659.1 cyclic-guanylate-specific phosphodiesterase PdeB -
APECO2_RS07850 1514838..1515308 - 471 WP_000136192.1 YlaC family protein -
APECO2_RS07855 1515424..1515975 - 552 WP_000102564.1 maltose O-acetyltransferase -
APECO2_RS07860 1516147..1516365 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
APECO2_RS07865 1516391..1516765 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
APECO2_RS07870 1517311..1520460 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
APECO2_RS07875 1520483..1521676 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T45681 WP_001291435.1 NZ_CP006834:c1516365-1516147 [Escherichia coli APEC O2-211]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T45681 NZ_CP006834:c1516365-1516147 [Escherichia coli APEC O2-211]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT45681 WP_000344800.1 NZ_CP006834:c1516765-1516391 [Escherichia coli APEC O2-211]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT45681 NZ_CP006834:c1516765-1516391 [Escherichia coli APEC O2-211]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References