Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 642187..642684 Replicon chromosome
Accession NZ_CP006834
Organism Escherichia coli APEC O2-211

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag APECO2_RS03345 Protein ID WP_001173343.1
Coordinates 642511..642684 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A1V2FLJ5
Locus tag APECO2_RS03340 Protein ID WP_001317561.1
Coordinates 642187..642483 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
APECO2_RS03315 638919..639638 - 720 WP_000611288.1 two-component system response regulator DcuR -
APECO2_RS03320 639635..641266 - 1632 WP_001216477.1 sensor histidine kinase -
APECO2_RS03325 641446..641676 + 231 WP_000371717.1 (4Fe-4S)-binding protein -
APECO2_RS03330 641688..641960 + 273 WP_000405647.1 N-acetyltransferase -
APECO2_RS03340 642187..642483 + 297 WP_001317561.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
APECO2_RS03345 642511..642684 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
APECO2_RS03350 642803..644320 - 1518 WP_000003805.1 lysine--tRNA ligase -
APECO2_RS03355 644557..646014 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T45677 WP_001173343.1 NZ_CP006834:642511-642684 [Escherichia coli APEC O2-211]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T45677 NZ_CP006834:642511-642684 [Escherichia coli APEC O2-211]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT45677 WP_001317561.1 NZ_CP006834:642187-642483 [Escherichia coli APEC O2-211]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMSFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT45677 NZ_CP006834:642187-642483 [Escherichia coli APEC O2-211]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAGTTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A1V2FLJ5

References