Detailed information of TA system TA045676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 294012..294607
• Replicon: chromosome
• Accession: NZ_CP006834
• Organism: Escherichia coli APEC O2-211

Toxin (Protein)

• Gene name: doc
• Uniprot ID: D6JG31
• Locus tag: APECO2_RS01470
• Protein ID: WP_000155159.1
• Coordinates: 294230..294607 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: D6JG32
• Locus tag: APECO2_RS01465
• Protein ID: WP_000557315.1
• Coordinates: 294012..294233 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APECO2_RS01440	289088..290197	+	1110	WP_001318085.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
APECO2_RS01445	290245..291171	+	927	WP_000003010.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
APECO2_RS01450	291168..292445	+	1278	WP_032284870.1	branched chain amino acid ABC transporter permease LivM	-
APECO2_RS01455	292442..293209	+	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
APECO2_RS01460	293211..293924	+	714	WP_000416895.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
APECO2_RS01465	294012..294233	+	222	WP_000557315.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
APECO2_RS01470	294230..294607	+	378	WP_000155159.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
APECO2_RS01475	294816..296132	+	1317	WP_000803191.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
APECO2_RS01480	296308..297195	+	888	WP_000099289.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
APECO2_RS01485	297192..298037	+	846	WP_000572164.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
APECO2_RS01490	298039..299109	+	1071	WP_000907828.1	sn-glycerol 3-phosphate ABC transporter ATP binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	291168..299849	8681

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (27-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13964.25 Da
• Isoelectric Point: 6.7263

>T45676 WP_000155159.1 NZ_CP006834:294230-294607 [Escherichia coli APEC O2-211]
MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNKYEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILAANPEFVELTVDVAAGKVSLEDIVTRLRQRGT

Nucleotide

• Length: 378 bp

>T45676 NZ_CP006834:294230-294607 [Escherichia coli APEC O2-211]
ATGACCATTCAGTTTATCTCAGCGGAGGAGATAATTTATTTCCACGATAAATTGCTTAGCGTTACACCAGGTGTAACAGG
AATGTCCGATCCAGGGCGAGCAGAAGCGCTTTTGTACCGGGTACTGAATAAGTATGAGTATGAAGGCGTTTCTGATCTTT
GGTTGCTGGCGGCGATGCATCTATTGGCAATCTCGCGCGGTCATATCTTTAATGATGGTAATAAGCGCACCGCACTGTTT
ATCACGCTATTATTCCTTAAACGTAACGGCATCATACTTGCTGCAAACCCAGAGTTTGTAGAATTGACGGTTGATGTGGC
GGCGGGAAAAGTATCGTTAGAAGATATCGTTACCCGCTTGCGTCAACGCGGTACCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8103.24 Da
• Isoelectric Point: 6.2168

>AT45676 WP_000557315.1 NZ_CP006834:294012-294233 [Escherichia coli APEC O2-211]
MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEFERYQKAKLDAEFDAIMAIHGSEIKELADK

Nucleotide

• Length: 222 bp

>AT45676 NZ_CP006834:294012-294233 [Escherichia coli APEC O2-211]
ATGCATACCATGAACTACAGCGAAGCGCGGCAGAACCTTGCATTAGTTCTGGAAACAGCGGCGACAGGTACTCCGGTAAC
GATCACGAGGCGTGGGCATAAGGCAGCTGTGATTATCAGCGCAGAAGAGTTTGAACGTTACCAGAAGGCTAAACTGGACG
CAGAATTTGACGCCATCATGGCTATTCATGGCAGCGAAATTAAGGAGTTAGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: D6JG31

Antitoxin

• AlphaFold DB: D6JG32