Detailed information of TA system TA045675

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 266736..267367
• Replicon: chromosome
• Accession: NZ_CP006834
• Organism: Escherichia coli APEC O2-211

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: L4J012
• Locus tag: APECO2_RS01310
• Protein ID: WP_001260301.1
• Coordinates: 266736..267011 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IZA1
• Locus tag: APECO2_RS01315
• Protein ID: WP_000593557.1
• Coordinates: 267008..267367 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APECO2_RS01295	261740..262807	+	1068	WP_000361470.1	HlyD family secretion protein	-
APECO2_RS01300	262804..265539	+	2736	WP_032284871.1	ribosome-associated ATPase/putative transporter RbbA	-
APECO2_RS01305	265539..266663	+	1125	WP_001318088.1	ABC transporter permease	-
APECO2_RS01310	266736..267011	+	276	WP_001260301.1	type II toxin-antitoxin system HicA family toxin	Toxin
APECO2_RS01315	267008..267367	+	360	WP_000593557.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
APECO2_RS01320	267487..267888	-	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
APECO2_RS01325	267894..268700	-	807	WP_000173650.1	nickel import ATP-binding protein NikE	-
APECO2_RS01330	268697..269461	-	765	WP_039025756.1	nickel import ATP-binding protein NikD	-
APECO2_RS01335	269461..270294	-	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
APECO2_RS01340	270291..271235	-	945	WP_000947063.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10185.87 Da
• Isoelectric Point: 10.6128

>T45675 WP_001260301.1 NZ_CP006834:266736-267011 [Escherichia coli APEC O2-211]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T45675 NZ_CP006834:266736-267011 [Escherichia coli APEC O2-211]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTT
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13389.28 Da
• Isoelectric Point: 5.1987

>AT45675 WP_000593557.1 NZ_CP006834:267008-267367 [Escherichia coli APEC O2-211]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLTNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT45675 NZ_CP006834:267008-267367 [Escherichia coli APEC O2-211]
ATGATCAAACTGAAGACACCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTACTAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: L4J012

Antitoxin

• AlphaFold DB: L4IZA1