Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4311598..4312216 Replicon chromosome
Accession NZ_CP006830
Organism Escherichia coli APEC O18

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag APECO18_RS21365 Protein ID WP_001291435.1
Coordinates 4311598..4311816 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag APECO18_RS21370 Protein ID WP_000344800.1
Coordinates 4311842..4312216 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
APECO18_RS21335 4306885..4307457 + 573 WP_000779831.1 YbaY family lipoprotein -
APECO18_RS21340 4307488..4307799 - 312 WP_000409908.1 MGMT family protein -
APECO18_RS21345 4308178..4308531 + 354 WP_000878140.1 DUF1428 domain-containing protein -
APECO18_RS21350 4308573..4310123 - 1551 WP_001350616.1 cyclic-guanylate-specific phosphodiesterase PdeB -
APECO18_RS21355 4310287..4310757 - 471 WP_000136192.1 YlaC family protein -
APECO18_RS21360 4310873..4311424 - 552 WP_000102543.1 maltose O-acetyltransferase -
APECO18_RS21365 4311598..4311816 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
APECO18_RS21370 4311842..4312216 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
APECO18_RS21375 4312761..4315910 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
APECO18_RS21380 4315933..4317126 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T45673 WP_001291435.1 NZ_CP006830:c4311816-4311598 [Escherichia coli APEC O18]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T45673 NZ_CP006830:c4311816-4311598 [Escherichia coli APEC O18]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT45673 WP_000344800.1 NZ_CP006830:c4312216-4311842 [Escherichia coli APEC O18]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT45673 NZ_CP006830:c4312216-4311842 [Escherichia coli APEC O18]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References