Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2126446..2127100 Replicon chromosome
Accession NZ_CP006830
Organism Escherichia coli APEC O18

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag APECO18_RS10740 Protein ID WP_000244781.1
Coordinates 2126446..2126853 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag APECO18_RS10745 Protein ID WP_000354046.1
Coordinates 2126834..2127100 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
APECO18_RS10720 2122403..2124136 - 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -
APECO18_RS10725 2124142..2124852 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
APECO18_RS10730 2124877..2125773 - 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
APECO18_RS10735 2125885..2126406 + 522 WP_001055874.1 flavodoxin FldB -
APECO18_RS10740 2126446..2126853 - 408 WP_000244781.1 protein YgfX Toxin
APECO18_RS10745 2126834..2127100 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
APECO18_RS10750 2127343..2128323 + 981 WP_000886078.1 tRNA-modifying protein YgfZ -
APECO18_RS10755 2128400..2129059 - 660 WP_000250274.1 hemolysin III family protein -
APECO18_RS10760 2129223..2129534 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
APECO18_RS10765 2129579..2131012 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T45666 WP_000244781.1 NZ_CP006830:c2126853-2126446 [Escherichia coli APEC O18]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T45666 NZ_CP006830:c2126853-2126446 [Escherichia coli APEC O18]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT45666 WP_000354046.1 NZ_CP006830:c2127100-2126834 [Escherichia coli APEC O18]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT45666 NZ_CP006830:c2127100-2126834 [Escherichia coli APEC O18]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References