Detailed information of TA system TA045625

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 1346963..1347614
• Replicon: chromosome
• Accession: NZ_CP006804
• Organism: Lactobacillus rhamnosus DSM 14870

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: K8Q4Y0
• Locus tag: N507_RS06590
• Protein ID: WP_005686631.1
• Coordinates: 1346963..1347346 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: K8QH19
• Locus tag: N507_RS06595
• Protein ID: WP_005686632.1
• Coordinates: 1347366..1347614 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N507_RS06575	1345999..1346526	-	528	WP_005686630.1	QueT transporter family protein	-
N507_RS06580	1346564..1346761	-	198	WP_064536013.1	hypothetical protein	-
N507_RS06590	1346963..1347346	-	384	WP_005686631.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
N507_RS06595	1347366..1347614	-	249	WP_005686632.1	antitoxin	Antitoxin
N507_RS06600	1347726..1348865	-	1140	WP_005686633.1	alanine racemase	-
N507_RS06605	1348852..1349226	-	375	WP_005686634.1	holo-ACP synthase	-
N507_RS06610	1349395..1350903	-	1509	WP_005686635.1	DEAD/DEAH box helicase	-
N507_RS06615	1351177..1352565	-	1389	WP_005686636.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13757.03 Da
• Isoelectric Point: 10.9764

>T45625 WP_005686631.1 NZ_CP006804:c1347346-1346963 [Lactobacillus rhamnosus DSM 14870]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Nucleotide

• Length: 384 bp

>T45625 NZ_CP006804:c1347346-1346963 [Lactobacillus rhamnosus DSM 14870]
ATGGATGTAACAGTCAAACGCGGGGATGTATTTTTCGCCGATTTATCGCCGGTTGTCGGGTCGGAACAAGGCGGCAACCG
GCCCGTACTTATTATTCAAAATAACGTTGGCAATCACTATAGTCCCACCGTCATTGTGGCTGCCATTACCTCCAAAATCC
AAAAACCCAAAATGCCCACTCATGTCGGTTTGCGCGCCAAGCAAGACGGGGTTGAGCGTAATTCAGTCATTTTGCTTGAA
CAGATTCGTACAATTGATAAGCAACGGTTACAGGCACGCGTTACCGCTCTTTCAAGTGCCAAAATGGCAGCGGTGGATCG
AGCACTGGCGATATCGGTCGGACTCGTCAGCTTGCCTAAGCCGAAAACATATAATAAAAACTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8936.13 Da
• Isoelectric Point: 4.4778

>AT45625 WP_005686632.1 NZ_CP006804:c1347614-1347366 [Lactobacillus rhamnosus DSM 14870]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Nucleotide

• Length: 249 bp

>AT45625 NZ_CP006804:c1347614-1347366 [Lactobacillus rhamnosus DSM 14870]
ATGAAGAAGGAAACGTCAGTAACGGTCGCGCTTGATAGTGCTATCAAAGCGCGTTTGGATGCCTATTGTGAGTTGAATGA
TATGGGTGTTCAGGATGTCATTACTGAAGCAGTGGCGAAGTTTTTGCAGTCACAAGCACCTGAGATCAATCAGTTGATTT
CGGGCTATGTGGCAATGGGACAAATCAATACGGAAATCAGCCATGCTTTCTCCGCCTGCGAAAGTGAGGCATACGCCCAT
ATTCGATAA

Structures

Toxin

• AlphaFold DB: K8Q4Y0

Antitoxin

• AlphaFold DB: K8QH19