Detailed information of TA system TA045624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafQ-relB/YafQ-DinJ
• Location: 519292..519872
• Replicon: chromosome
• Accession: NZ_CP006803
• Organism: Lactobacillus gasseri DSM 14869

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: A0A249NKC7
• Locus tag: N506_RS02590
• Protein ID: WP_003647258.1
• Coordinates: 519292..519597 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A249NIV0
• Locus tag: N506_RS02595
• Protein ID: WP_021314843.1
• Coordinates: 519597..519872 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N506_RS02545	515015..515497	+	483	WP_095669626.1	nitroreductase family protein	-
N506_RS02550	515544..517121	-	1578	WP_095669627.1	ABC transporter ATP-binding protein/permease	-
N506_RS02555	517260..517595	+	336	WP_095669628.1	hypothetical protein	-
N506_RS02560	517595..518041	+	447	WP_095669629.1	GNAT family N-acetyltransferase	-
N506_RS02565	518048..518350	+	303	WP_095669630.1	hypothetical protein	-
N506_RS02570	518343..518702	+	360	WP_095669631.1	hypothetical protein	-
N506_RS02575	518709..518918	+	210	WP_095669632.1	DUF3923 family protein	-
N506_RS02580	518937..519098	+	162	Protein_498	Fic family protein	-
N506_RS02590	519292..519597	-	306	WP_003647258.1	type II toxin-antitoxin system YafQ family toxin	Toxin
N506_RS02595	519597..519872	-	276	WP_021314843.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
N506_RS02600	520204..520737	+	534	WP_095669633.1	GNAT family N-acetyltransferase	-
N506_RS02605	520740..521222	+	483	WP_095669634.1	GNAT family N-acetyltransferase	-
N506_RS02615	521490..522107	+	618	Protein_503	NUDIX hydrolase	-
N506_RS02620	522135..523328	+	1194	WP_095669636.1	MFS transporter	-
N506_RS02625	523345..523809	+	465	WP_095669637.1	NUDIX hydrolase	-
N506_RS02630	523824..524588	+	765	WP_095669638.1	nucleoside phosphorylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-97)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-88)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11985.87 Da
• Isoelectric Point: 10.2029

>T45624 WP_003647258.1 NZ_CP006803:c519597-519292 [Lactobacillus gasseri DSM 14869]
MAQINYTPQFKRDYKKLKRKHYDLNKLKNVIQLLIDEKFKILVDKYDDHQLKGSLRSLRALHVEHNKAGNWILVYKIHSG
NLELLTIDLVSTGSHDHTYRT

Nucleotide

• Length: 306 bp

>T45624 NZ_CP006803:c519597-519292 [Lactobacillus gasseri DSM 14869]
ATGGCTCAAATTAACTACACTCCTCAATTTAAGCGAGATTATAAGAAGCTTAAAAGAAAACATTATGATTTGAATAAATT
AAAAAATGTCATTCAATTACTTATTGACGAAAAATTTAAAATTCTTGTTGATAAATATGATGACCATCAACTAAAAGGTT
CTTTAAGGTCACTACGTGCATTACATGTTGAGCATAATAAAGCTGGTAATTGGATTTTAGTTTATAAAATTCATAGCGGC
AATTTAGAACTGTTAACCATCGACTTGGTTTCTACTGGTTCCCACGATCATACTTATAGAACATAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10461.79 Da
• Isoelectric Point: 4.8096

>AT45624 WP_021314843.1 NZ_CP006803:c519872-519597 [Lactobacillus gasseri DSM 14869]
MTTTVSLRLPEDLKKQATDVFNEYGLDWSSAMRMILTQVVSENAIPVNLHRYSEISPSMKSNIDRSLQEYKAGNYKTTNS
VKELFEELDKD

Nucleotide

• Length: 276 bp

>AT45624 NZ_CP006803:c519872-519597 [Lactobacillus gasseri DSM 14869]
ATGACAACTACCGTAAGTTTAAGATTACCAGAAGATTTAAAGAAACAAGCAACAGATGTATTCAACGAATACGGTCTCGA
TTGGTCATCTGCAATGCGAATGATCTTAACTCAAGTCGTAAGTGAAAATGCAATTCCTGTTAATTTACATAGATATTCTG
AAATTTCTCCTTCGATGAAATCAAATATTGATAGATCACTTCAAGAATACAAAGCAGGAAATTACAAAACCACAAACTCA
GTAAAAGAGCTATTTGAGGAGTTAGATAAAGACTAA

Structures

Toxin

• AlphaFold DB: A0A249NKC7

Antitoxin

• AlphaFold DB: A0A249NIV0